摘要
该文建立了免疫亲和柱净化-超高效液相色谱串联质谱法测定啤酒中FB1、FB2和FB3的含量。与液相色谱相比较，无需衍生化，避免了化学衍生法受衍生产物的不稳定性、衍生剂的浓度、温度和反应时间等衍生效率的影响。采用多反应监测（MRM）方式进行检测，3种物质在2．5～100ng／mL范围具有良好的线性关系，相关系数（R^2）均大于0．999，在空白样品中加入1．0μg/kg和8．0μg／kg两个浓度水平的伏马毒素，平均回收率为88．3％-95．1％，相对标准偏差（RSD）在4．5％～8．1％，3种物质定量限均低于0．6μg/kg，精密度试验结果相对标准偏差（RSD）为0．9％～1．7％。该方法准确度高、精密度好，适用于啤酒中伏马毒素含量的测定，可为啤酒中伏马毒素的风险评估数据来源提供参考依据。
A method for FB1, FB2 and FB3 determination in beer was established by immune affinity column purification-ultra performance liquid chromatography tandem mass spectrometry. Compared with liquid chromatogram, the method avoided the effects of derivative efficiency （including derivatives instability, derivating agent concentration, temperature, reaction time, etc.） on chemical derivatization. By multiple reaction monitoring mode for detection, three kinds of substances had a good linear relationship in 2.5-100 ng/ml range, and the correlation coefficient R^2 were all more than 0.999. Adding fumonisin 1.0μg/kg and 8.0 μg/kg into the blank samples, results showed that the average recovery rate was 88.3%-95.1%, RSD was 4.5%-8.1%, limit of quantitation were all below 0.6 μg/kg. RSD of precision experiments was 0.9%-1.7%. The method was with high accuracy and good precision, and it was suitable for fumonisin contents determination in beer, which could provide a reference basis for risk assessment data sources of fumonisin in beer.
出处
《中国酿造》
CAS
北大核心
2016年第1期152-155,共4页
China Brewing
基金
贵州省科学技术基金项目(黔科合J字[2009]2240)
