摘要
目的探讨血小板数量对血小板聚集率检测的影响,提高检测质量,保证聚集率测定结果的可靠性。方法收集211例健康人静脉全血标本,离心获取富血小板血浆（platelet-rich plasma,PRP）和乏血小板血浆（platelet-poor plasma,PPP）,将PRP用自身PPP梯度稀释后获取不同血小板浓度的PRP检测样本,并确认PRP血浆中血小板浓度;利用血浆比浊法测定血小板聚集率,讨论血小板数量与血小板聚集率之间的相关性。结果在二磷酸腺苷（a denosine diphosphate,ADP）和花生四烯酸（arachidonic acid,AA）诱导下,血小板聚集率均在实验室设定参考范围之内,随着血小板浓度的降低,聚集率显著下降（P〈0.05）;当浓度降低至〈95×109L时,聚集率检测结果低于既定参考范围;血小板90~350×109L与聚集率呈正相关（r_（AA）=0.67,r_（ADP）=0.69）,其他浓度组与聚集率无相关性。结论血小板浓度对聚集率有影响,聚集率测定应在浓度为95×109L以上时测定,浓度与聚集率正相关,最能准确反映聚集率。
Objective To investigate the effect of platelet counts on platelet aggregation test,to regulate platelet aggregation rate detection to improve detection quality,in order to ensure the reliability of measurements of platelet aggregation. Methods 211 cases of healthy people venous whole blood samples were included and centrifuged to obtain platelet-rich plasma（ platelet-rich plasma,PRP） and platelet-poor plasma（ platelet-poor plasma,PPP）,the PRP with PPP got different dilutions of platelet concentration of PRP samples tested,concentration of PRP platelet were confirmed in plasma; Platelet aggregation rate was detected by platelet aggregation nephelometry,and discuss the correlation with platelet. Results Adenosine diphosphate（ ADP） and arachidonic acid（ AA） induced platelet aggregation rate within a laboratory setting reference range. with decreasing concentration of platelet,the aggregation rate decreased significantly（ P〈0. 05）; when the concentration was reduced to 95 × 109/ L,the aggregate rate of the test resulted below the established reference range; platelet 90 ~ 350 × 109/ L was significantly correlation with the accumulation（ rAA= 0. 67,rADP= 0. 69）,other concentrations and aggregation had no correlation. Conclusion Platelet concentration can affect platelet aggregation rate,determination of aggregation rate should be limited at above of 95 × 109/ L,which reflect the relation of concentration and aggregation rate,and accurately reflect the concentration of aggregation.
出处
《四川医学》
CAS
2015年第12期1721-1724,共4页
Sichuan Medical Journal
关键词
血小板浓度
富血小板血浆
血小板聚集率
血浆比浊法
血小板聚集测定仪
platelet concentration
platelet-rich plasma
platelet aggregation
plasma turbidimetry
platelet aggregation analyzer
