摘要
以玫瑰香葡萄为实验试材,研究洋葱霍尔德氏菌(Burkholderia contaminans B-1)对玫瑰香葡萄采后灰霉病的抗性诱导机理,对经拮抗菌处理贮藏后果实的腐烂率及其抗性相关物质进行测定。结果表明:拮抗菌B.contaminans B-1可有效降低玫瑰香葡萄采后腐烂率,与对照相比,果梗腐烂率降低69.58%,果肉腐烂指数降低63.93%。拮抗菌处理对果实苯丙烷代谢途径、活性氧代谢途径和其他抗性相关酶类活性均有影响。与对照相比,B.contaminans B-1处理可提高葡萄果实苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、超氧化物歧化酶(SOD)和多酚氧化酶(PPO)活性,促进过氧化氢(H_2O_2)含量积累,延缓过氧化氢酶(CAT)活性峰值出现的时间。此外,拮抗菌处理也可以诱导果实病程相关蛋白几丁质酶(CHI)和β-1,3葡聚糖酶(GLU)活性的上升。
In this study, the mechanism by which the antagonistic bacterium Burkholderia contaminans B-1 induces resistance to Botrytis cinerea in postharvest Muscat grape berry was investigated. The results showed that B. contaminans B-1 reduced significantly the rot rate of postharvest grape berries. Compared with the control, the rot rates of the stem and fruit treated with B. contaminans were decreased by 69.58% and 63.93%, respectively. B. contaminans B-1 could affect the phenylpropanoid metabolic pathway, reactive oxygen species metabolic pathway, and the activities of resistance-related enzymes. Treatment with B. contaminans B-1 enhanced the activities of phenylalanine ammonia-lyase（PAL）, peroxidase（POD）, superoxide dismutase（SOD） and polyphenloxidase（PPO）. B. contaminans B-1 also increased hydrogen peroxide（H_2O_2） content and delayed the peak time of catalase（CAT） activity, and enhanced the activities of resistance-related enzymes, chitinase（CHI） and β-1,3-glucanase（GLU）, in grapes.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2016年第2期266-270,共5页
Food Science
基金
山西省农业科学院博士后基金项目(BSH-2015JJ-003)
山西省农业科学院自主创新项目(2015ZZCX-16)
国家自然科学基金面上项目(31371868)
关键词
玫瑰香葡萄
生物防治
抗性诱导
Muscat grape
biological control
induced disease resistance
