摘要
[目的]研究不同含水量对溪荪鸢尾种子保存效果的影响。[方法]以寒地特色野生种溪荪鸢尾种子为材料,通过硅胶干燥法获得不同含水量的种子,在液氮中保存24 h后,采用水浴化冻、自来水水冲化冻和室温化冻3种化冻方式,检测不同条件下种子发芽率来检验保存效果。[结果]种子含水量为5.4%～7.2%时的干燥处理显著提高了种子发芽率,但含水量降到3.2%时会降低种子发芽率;种子含水量为7.2%时超低温保存后萌发率会显著提高,水浴化冻最适。[结论]最佳种子含水量为7.2%,保存后萌发率高达98.70%。
[Objective] To study the effects of different water content on preservation of Iris sanguinea seed.[Method]There is no denying that liquid nitrogen cryopreservation is an ideal way to preserve germplasm for a long time. When using seeds as saving materials,the optimal moisture contents and thawed are very important to the effect of cryopreservation. This study uses the wild Iris sanguinea seeds for the experimental materials which are grow in cold places. We get the seeds of different water contents with the method of drying by silica gel,and then seeds were preserved in liquid nitrogen for 24 hours. Using three kinds of defrost modes to test the seed germination rate under different conditions to test effect.[Result]The results showed that seed moisture content was 5. 4%- 7. 2% of dry treatment had significant effect on seed germination rate,but will reduce the seed germination rate under 3. 2% moisture content. Seed germination rate can significantly improve after cryopreservation when the moisture content was 7. 2%.[Conclusion]The germination rate is as high as 98. 70% when the optimum water content is 7. 2%.
出处
《安徽农业科学》
CAS
2015年第36期230-231,233,共3页
Journal of Anhui Agricultural Sciences
基金
大学生国家级创新项目(201410225050)
中央高校基本科研业务费专项(2572015BA07)
关键词
溪荪鸢尾
种子
超低温保存
Iris sanguinea
Seeds
Cryopreservation
