摘要
探讨复合红景天苷微球的胶原蛋白材料支架对大鼠骨髓间充质干细胞的影响及作用。将红景天苷制作成微球复合到胶原蛋白中,扫描电镜观察材料支架的表征,接种大鼠骨髓间充质干细胞后扫描电镜观察细胞与材料的黏附性,CCK-8法测细胞在材料上的增殖情况,HE染色检测细胞在材料上的增殖及形态,S100免疫荧光化学法检测干细胞向神经细胞的分化情况。结果显示,细胞接种到材料上,在材料上黏附并生长。同种材料,随着时间的延长,材料上的细胞显著增殖(P<0.05)。神经细胞标志性蛋白S100表达为阳性。复合红景天苷微球的胶原蛋白具有良好的生物相容性,红景天苷微球不影响细胞在材料上的增殖,且可有效诱导大鼠间充质干细胞向神经细胞分化。
The objective of this work is to investigate the effect of collagen scaffolds combined with salidroside microspheres on rat bone mesenchymal stem cells（r BMSCs）. The salidroside was made into microspheres that combined into the collagen. The surface character of material was observed by scanning electron microscopy（SEM）. The material was implanted with r BMSCs and observed for the cell adhesion on the material by SEM. The cell proliferation on the material was measured by CCK-8, and the cell proliferation and morphology on the material were detected by HE staining. S100 immunofluorescence was used to detect the differentiation of stem cells into nerve cells. Cells were seeded onto the scaffold, adhered and grew well on materials. While the time increased, the cells on the same material were significant proliferation（P 0.05）. The expression of nerve cell marker protein S100 was positive. Conclusively, collagen combined with salidroside microspheres had good biocompatibility, salidroside microspheres did not affect cell proliferation on the material, and it could induce effectively r BMSCs into nerve cells.
出处
《生物技术通报》
CAS
CSCD
北大核心
2016年第1期207-212,共6页
Biotechnology Bulletin
基金
国家自然科学基金面上项目(81470087)
