摘要
目的探讨通督调神针灸预处理对脑缺血再灌注大鼠神经行为功能和脑水含量的影响及mi RNA664、MMP9表达的调控作用机制。方法将130只Wistar大鼠随机分为A组(电针预处理组)30只、B组(艾灸预处理组)30只、C组(阿司匹林预处理组)30只、D组(模型组)30只和E组(空白对照组)10只。A组进行电针治疗;B组用清艾条悬灸;C组用阿司匹林10 mg/kg灌胃。治疗7 d后各组进行脑缺血再灌注模型制作,于再灌注后24 h,观察大鼠神经行为功能和脑水含量以及皮质区相关mi RNA、MMP9的表达。结果 A组、B组、C组和D组神经行为学评分与E组比较,差异均具有统计学意义(P<0.01)。A组、B组神经行为学评分与C组比较,差异均具有统计学意义(P<0.01)。A组神经行为学评分与B组比较,差异具有统计学意义(P<0.01)。A组、B组、C组和D组脑水含量、mi RNA664相对表达量及MMP9相对表达量与E组比较,差异均具有统计学意义(P<0.01,P<0.05)。A组、B组和C组脑水含量、mi RNA664相对表达量及MMP9相对表达量与D组比较,差异均具有统计学意义(P<0.01)。A组和B组脑水含量、mi RNA664相对表达量及MMP9相对表达量与C组比较,差异均具有统计学意义(P<0.01,P<0.05)。A组脑水含量、mi RNA664相对表达量及MMP9相对表达量与B组比较,差异具有统计学意义(P<0.01,P<0.05)。结论通督调神针灸预处理可有效降低脑缺血再灌注大鼠的神经行为学评分和脑水含量,并通过调控mi RNA664的表达降低MMP9相对表达量。通督调神针灸预处理脑保护机制之一可能通过调控mi RNA664的表达降低MMP9相对表达量,诱导脑缺血耐受,减轻脑水肿。
Objective To investigate the effect of meridian-unblocking and mind-regulating acupuncture pretreatment on neurobehavioral functions and cerebral water content and explore its mechanism for modulating mi RNA664 and MMP9 expressions in cerebral ischemia/reperfusion rats. Methods One hundred and thirty Wistar rats were randomized into group A（electroacupuncture pretreatment） of 30 rats, group B（moxibustion pretreatment） of 30 rats, group C（aspirin pretreatment） of 30 rats, group D（model） of 30 rats and group E（blank control） of 10 rats. Group A received electroacupuncture; group B, suspended moxibustion with moxa sticks; group C, an oral gavage of aspirin 10 mg/kg. A model of cerebral ischemia/reperfusion was made in every group after seven days. Rat neurobehavioral functions were observed and cerebral water content and relative mi RNA664 and MMP9 expressions in the cortical region were determined at 24 hours after reperfusion. Results There was a statistically significant difference in the neurobehavioral score between group A, B, C or D and group E（P〈0.01）, between group A or B and group C（P〈0.01） and between groups A and B（P〈0.01）. There were statistically significant differences in cerebral water content and relative mi RNA664 and MMP9 expressions between group A, B, C or D and group E（P〈0.01,P〈0.05）, between group A, B or C and group D（P〈0.01）, between group A or B and group C（P〈0.01,P〈0.05） and between groups A and B（P〈0.01,P〈0.05）. Conclusions Meridian-unblocking and mind-regulating acupuncture pretreatment can effectively decrease the neurobehavioral score and cerebral water content and reduce relative MMP9 expression through modulating mi RNA664 expression in cerebral ischemia/reperfusion rats. One of the mechanisms of Du meridian-unblocking and mind-regulating acupuncture pretreatment for protecting the brain may be reducing relative MMP9 expression, inducing tolerance to cerebral ischemia and relieving cerebral edema by the modulation of mi RNA664 expression.
出处
《上海针灸杂志》
2016年第1期76-80,共5页
Shanghai Journal of Acupuncture and Moxibustion
基金
安徽省自然科学基金项目(1308085MH174)
关键词
针灸疗法
通督调神
MI
RNAS
MMP9
再灌注损伤
大鼠
电针
Acupuncture therapy
Unblocking Du meridian and regulating mind
mi RNAs
MMP9
Reperfusion injury
Rats
Electroacupuncture
