摘要
对青扦针叶总黄酮超声提取工艺和抗氧化性进行了研究,在单因素试验基础上,采用响应面法优化青扦针叶总黄酮的提取工艺,并通过对DPPH·、ABTS·的清除试验以及与Vc、槲皮素和芦丁的总还原力进行比较,来研究青扦总黄酮的抗氧化活性。结果表明,在料液比为1∶20的条件下,得到黄酮的最佳提取工艺为:乙醇体积分数50.63%、超声功率319.63 W、超声时间49.46min、超声温度为64.88℃,此时青扦黄酮的提取率可达4.118 69%。青扦针叶总黄酮对DPPH·清除率可达90.21%,清除ABTS·的Trolox当量为29 633.3μmol·g^(-1),其还原能力优于芦丁,但不及Vc和槲皮素。表明青扦针叶总黄酮具有较强的抗氧化能力,可为青扦针叶黄酮资源的开发利用提供依据。
The optimal ultrasound assisted extraction conditions of total flavonoids from Picea wilsonii nee- dles were investigated, in which based on the single-factor tests, the optimal values were determined by re- sponse surface analysis. Antioxidant activity of the extract was measured. The results showed that with the ratio of material to solvent 1 ： 20,the optimum extraction conditions were ethanol concentration： 50.63% （V/V） ,power of the generator of the ultrasound of. 319.63 W,time of ultrasound assisted extraction 49.46 min,temperature 64.88℃ ,the ultrasonic power 319.63 W,and ethanol volume fraction 50.63% ,by which the extraction rate of total flavonoids was 4. 118 69%. The scavenging rate of the extract on DPPH · was 90.21% and the equivalent ABTS scavenging rate was 29 633.3 μmol · g^-1, and the reducing power was better than that of rutin,but not as good as Vc and quercetin,indicating that the antioxidant effects of the total flavonoids in the needles of P. wilsonii was good. The results could be provide references for the de- velopment and utilization of the needles of P. wilsonii.
出处
《西北林学院学报》
CSCD
北大核心
2016年第1期243-249,共7页
Journal of Northwest Forestry University
关键词
青扦针叶
总黄酮
超声提取
响应面法
Picea wilsonii
total flavonoid
ultrasonic extraction
response surface methodology
