摘要
以石墨烯量子点(GQDs)为还原剂和稳定剂在其表面原位生长银纳米粒子(AgNPs),制备了具有良好分散性的GQDs/AgNPs纳米复合物,其粒径小于30 nm。GQDs/AgNPs纳米复合物具有类过氧化物酶的催化活性能有效催化H_2O_2氧化3,3',5,5'-四甲基联苯胺(TMB)并发生显色反应。稳态动力学分析表明GQDs/AgNPs催化动力学遵循典型的Michaelis-Menten模型,其催化机理符合乒乓机制。与辣根过氧化物酶(HRP)相比,GQDs/AgNPs纳米复合物具有更强的亲和性。基于GQDs/AgNPs的催化活性和葡萄糖氧化产生H_2O_2的原理建立了H_2O_2和葡萄糖的比色检测方法,检出限分别为0.18和1.6μmol/L。将本方法应用于血浆中葡萄糖的检测分析,结果与标准方法相符。
Graphene quantum dots/Ag nanoparticles nanocomposite（GQDs/AgNPs） was prepared via in-situ growth of AgNPs on the surface of GQDs,in which GQDs served as both reducing agent and stabilizer.The as-prepared nanocomposites were mono-dispersed with an average diameter of less than 30 nm.The obtained GQDs@ AgNPs nanocomposites exhibited excellent intrinsic peroxidese-like activity,which could catalyze the oxidization of 3,3＇,5,5＇-tetramethylbenzidine（TMB） by H2O2 to produce a colour product.Steady-state kinetic assays showed that the catalytic activity of GQDs/AgNPs towards H2O2 fitted well with typical Michaelis-Menten kinetic model,followed by the ping-pong mechanism.Compared with the horseradish peroxidase（HRP）,GQDs/AgNPs showed higher affinity towards TMB and H2O2 substrate.Based on the intrinsic peroxidese-like activity of GQDs/AgNPs nanocomposites and the production of H2O2 after the oxidation of glucose,a colorimetric method was developed for the detection of H2O2 and glucose,along with detection limit of 0.18 μmol/L and 1.6 μmol/L,respectively.The present method was applied to the detection of glucose in human serum,and the obtained results agreed with that obtained by reference method.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2016年第1期41-48,共8页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金项目(Nos.21275027,21235001,21475017)资助
