摘要
为了揭示富亮氨酸重复类受体蛋白激酶(LRR-RLKs)家族成员OsLPR1在水稻生长发育与抗逆性中的功能,利用反向遗传学方法构建了OsLPR1的RNAi载体,将其导入野生型水稻中获得转基因植株,观察表型并且分析OsLPR1基因在野生型和转基因植株中的表达情况.在表型观察中发现RNAi转基因植株出现白化苗.进一步的RT-PCR分析发现,白化苗中目的基因OsLPR1的表达量明显偏低,野生型中的表达量则较高,这表明OsLPR1基因表达异常会导致水稻出现白化现象.另外,还分析了OsLPR1的组织特异性表达,结果表明,OsLPR1在不同的组织器官中均有表达,但在叶片及叶鞘中高表达,这说明该基因可能与水稻叶片的生长发育有关.
To reveal the function of OsLPR1, a member of leucine-rich repeat receptor protein-like kinase family （LRR- RLKs）, in growth and development of rice, the RNAi vector of OsLPR1 were constructed to produce transgenic plants by reverse genetics method. It was found that knockdown of OsLPR1 produced the albino seedlings by phenotypic observation. RT-PCR expression analysis showed that the expression of OsLPR1 was obviously lower in the albino seedlings compared with that in wild type, which suggested that OsLPR1 RNAi lead to the rice albino. In addition, the tissue-specific expres- sion of OsLPR1 was analyzed, and the results showed that OsLPR1 was expressed in different organs, but was higher in the leaf and leaf sheath. Overall, the result indicated that OsLPR1 may be relative to the leaf development in rice.
出处
《天津师范大学学报（自然科学版）》
CAS
2016年第1期48-51,共4页
Journal of Tianjin Normal University：Natural Science Edition
基金
国家自然科学基金资助项目(31171515)
天津市高校中青年骨干创新人才培养计划资助项目(ZX110gg017)
