摘要
目的探讨医用臭氧治疗特发性肺纤维化的可行性。方法将40只清洁级雄性wistar大鼠随机分为试验组和对照组。所有大鼠采用气管内滴入博来霉素的方法制造肺纤维化模型,对照组大鼠隔日腹腔内注射生理盐水,实验组大鼠隔日腹腔内注射医用臭氧。于第28 d每组随机选取10只检测肺功能后处死,取右肺制作组织切片观察肺纤维化程度,取左肺检测超氧化物歧化酶及羟脯氨酸含量。每组剩余10只观察生存时间。结果对照组大鼠各肺功能指标均较实验组明显下降(P<0.05)。对照组大鼠肺纤维程度较实验组严重,肺纤维化程度评分显著高于实验组[(1.9±0.5)分比(1.2±0.4)分,p<0.05]。实验组大鼠肺组织中超氧化物歧化酶含量较对照组明显升高[(208.48±29.37)U·mg^(-1)·pro^(-1)比(163.34±21.42)U·mg^(-1)·pro^(-1),P<0.05],羟脯氨酸的含量较对照组明显降低[(2.25±0.28)mg/g比(2.68±0.37)mg/g,P<0.05]。实验组大鼠中位生存期显著长于对照组(79 d比59 d,P<0.05)。结论医用臭氧能延缓大鼠肺纤维化的进展。
Objective To explore the feasibility of medical ozone in treatment of pulmonary fibrosis.Methods Forty Wistar rats were randomly divided into an experimental group and a control group, with 20 rats in each group. All rats were intratreacheally instilled with bleomycin to induce pulmonary fibrosis. Then the rats were intraperitoneally injected with physiological saline every other day in the control group, and with medical ozone every other day in the experimental group. After 28 days,10 rats in each group were sacrificed after lung function test. Right lung tissues were sampled for pathological examination, and left lung tissues were sampled for measurement of superoxide dismutase （ SOD） and hydroxyproline. The remaining 10 rats in each group continued to be normally fed and intraperitoneally injected for observation of the survival time. Results The lung function of the control group significantly decreased compared with the experimental group. The degree of lung fibrosis in the control group was more severe than that in the experimental group （ lung fibrosis score： 1. 9 ±0. 5 vs. 1. 2 ±0. 4, P 〈 0. 05） . The level of SOD in lung tissue was significantly higher and the level of hydroxyproline was significantly lower in the experimental group compared with the control group [ （ 208. 48 ± 29. 37 ） U · mg^-1·pro^-1 vs. （ 163. 34 ± 21. 42） U·mg^-1 ·pro^-1 , （ 2. 25 ±0. 28） mg/ g vs. （ 2. 68 ±0. 37） mg/ g, P 〈 0. 05] . The rats in the experimental group had longer survival time compared with the control group （ 79 d vs. 59 d, P 〈 0. 05） .
Conclusion Medical ozone can delay the progress of pulmonary fibrosis in rats.
出处
《中国呼吸与危重监护杂志》
CAS
北大核心
2016年第1期39-42,共4页
Chinese Journal of Respiratory and Critical Care Medicine
关键词
肺纤维化
医用臭氧
超氧化物歧化酶
羟脯氨酸
Pulmonary fibrosis
Medical ozone
Superoxide dismutase
Hydroxyproline
