摘要
目的:探讨mi R-519参与调节胃癌细胞活力的机制。方法:q PCR检测胃癌细胞中mi R-519表达,Western印迹法检测胃癌细胞中Hu R蛋白表达,M法研究肿瘤细胞的活力,脂质体转染法分别转染Hu R真核表达质粒及干扰片段、转染mi R-519。结果:胃癌细胞株中Hu R蛋白表达高于对照组细胞,过表达Hu R可增加细胞活力(P<0.001),干扰Hu R表达后,细胞活力降低(P=0.001);胃癌组织中mi R-519表达低于对照组(P=0.001),过表达mi R-519可抑制Hu R蛋白表达并降低胃癌细胞活力。结论:Mi R-519可能通过下调Hu R蛋白表达而降低胃癌细胞活力。
Objective： To investigate the role of miR-519 in regulating the cell activity of gastric cancer. Methods： The expression of miR-519 was measured by qPCR. The protein expression of HuR was detected by Western blot. MTT assay was used to detect the cell activity of gastric cancer cells. Results： The protein level of HuR in the gastric cancer cells was higher than that in the control cells. Over-expression of HuR resulted in the increase in the cell activity of gastric cancer （P〈0.001）. Suppression of HuR decreased the cell activity of gastric cancer （P=0.001）. The miR-519 level in the gastric cancer cells was lower than that in the control cells （P=0.001）. Over-expression of miR-519 decreased the protein level of HuR and the activity of gastric cancer cells. Conclusion： MiR-may inhibit the gastric cancer cell activity through suppression of HuR expression.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2016年第1期19-23,共5页
Journal of Central South University ：Medical Science
基金
新疆医科大学科研创新基金(XJC201384)~~
