摘要
目的：建立祛湿通脉丸中绿原酸含量的测定方法。方法：采用高效液相色谱法,色谱柱：InertSustain-C_（18）柱（4.6mm×150mm,5μm）;流动相：乙腈-0.4%磷酸溶液（11∶89）;流速：1.0mL/min;检测波长：327nm。结果：绿原酸进样量在0.03957~0.7914μg范围内与峰面积呈良好线性关系（r=0.999 9）,绿原酸平均加样回收率为99.95%,RSD为1.09%（n=6）。结论：该方法简单、精确、可靠,可用于祛湿通脉丸的质量控制。
Objective：To evaluate the determination of Chorogenic acid in Qushitongmai Pills by HPLC. Methods：Using high performance liquid chromatography （HPLC） method, InertSustain-C18 column （4.6）〈 150mm, 5μm）, acetonitrile 0.4 M ortho-phosphoric（11：89） as mobile phase, flow rate of 1.0mL/min, detection wavelength of 327nm. Results： The linear range was 0. 03957- 0. 7914wg and correlation coefficient was 0. 9999. The average recovery and the relative standard deviation were 99.95 % and 1.09 （n= 6） respectively. Conehlsion： The method is simple, accurate and reliable, which can be applied to the determination of Chorogenic acid in Qushitongmai Pills.
出处
《亚太传统医药》
2016年第3期37-38,共2页
Asia-Pacific Traditional Medicine
