摘要
目的验证应用透视窗式乳糖胆盐发酵培养基管野外现场检测生活饮用水中大肠杆菌的可行性。方法参照《生活饮用水标准检验方法》(GB/T 5750.12-2006)中多管发酵法,用透视窗式乳糖胆盐发酵培养基管与小导管式乳糖蛋白胨培养液对实验室配置的加标水样和野外环境采集的水样进行比较。结果经统计分析,两种培养液对加标水样和环境水样检测结果无统计学差异,其中加标水样的统计结果为P=0.539(P>0.05)、环境水样的统计结果为P=0.772(P>0.05)。结论透视窗式乳糖胆盐发酵培养基管操作简便实用,稳定性好,可以用于生活饮用水样品的检测,尤其适合在野外现场应用。
Objectives To validate the feasibility of a method using lactose peptone broth in a kind of fermentation tube with observable cassette to detect Escherichia coli in water on wild fields. Methods Comparing the multiple- tube fermentation method used in the standard examination methods for drinking water with the lactose peptonein the observable- cassette fermentation tube method,Escherichia coli was test in water samples,including standard water samples prepared in laboratory and water samples collected from wild fields. The lactose peptone broth used in the multiple- tube fermentation method was prepared according to the national criteria; and the lactose peptone broth used in the observable- cassette fermentation tube method was from commercial kits. Results No significant difference was observed between the results in the observable- cassette fermentation tube method and the multi- tube fermentation method for standard water samples made in laboratory（ t = 0. 539; P 0. 05） or water samples collected from outdoors（ t = 0. 772; P 0. 05）. Conclusion The observable- cassette fermentation tube method was easy to carry out and stable,which can be used to detect bacteria in drinking water,especially for performing test in outdoors.
出处
《环境卫生学杂志》
2015年第6期545-546,551,共3页
JOURNAL OF ENVIRONMENTAL HYGIENE
关键词
大肠菌群
乳糖蛋白胨
透视窗
野外现场
Escherichia coli
lactose peptone broth
observable-cassette
wild field
