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桂枝化合物的分离与鉴定及神经保护作用 被引量:23

Isolation and identification of chemical constituents from twigs of Cinnamomum cassia Presl(Lauraceae)and their neuroprotective activities
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摘要 目的研究桂枝的化学成分及其神经保护作用。方法运用多种色谱方法对桂枝体积分数90%乙醇提取物的化学成分进行分离纯化,并根据波谱数据和理化性质确定其化学结构。采用建立体外脂多糖(lipopolysaccharide,LPS)激活N9小胶质细胞异常活化的筛选模型对所分离化合物的活性进行评价。结果分离得到了10个化合物,分别鉴定为桂皮醛(cinnamaldehyde,1)、肉桂酸(cinnamic acid,2)、肉桂醇(cinnamyl alcohol,3)、原儿茶醛(protocatechuic aldehyde,4)、原儿茶酸(protocatechuic acid,5)、香豆素(coumarin,6)、钩果草苷(harpagoside,7)、二氢肉桂苷(dihydrocinnacasside,8)、3,4,5-三甲氧基-1-β-D-吡喃葡萄糖苷(3,4,5-trimethoxypheny-1-β-Dglucopyranoside,9)和邻香豆酸葡萄糖苷(O-coumaric acid glucoside,10)。抑制活性试验表明,化合物3能够较好地抑制LPS刺激的N9小胶质细胞NO释放,在其发挥抑制作用的浓度范围内不影响小胶质细胞的存活率。结论从桂枝乙醇提取物中分离得到10个化合物,其中化合物7、9和10为首次从樟科植物中分离得到。化合物3具有较好的抑制活性。 Objective To study the chemical constituents isolated from twigs of Cinnamomum cassia and their neuroprotective activities. Methods A variety of column chromatographic techniques were used for the isolation of chemical constituents. Their structures were identified on the basis of spectral data. The effects of target compounds on the NO production in vitro were evaluated using lipopolysaccharide (LPS)-activated N9 microglial cells. Results Ten compounds were isolated and identified as cinnamaldehyde (1), cinnamic acid (2), cinnamyl alcohol (3), protocatechuic aldehyde (4), protocatechuic acid ( 5 ), coumarin ( 6 ), harpagoside (7) , dihydrocinnacasside ( 8 ) , 3,4,5-trimethoxypheny-1 -β-D-glu-copyranoside ( 9 ) , and O-coumaric acid glucoside (10). Among them, compound 3 moderately suppressed NO production induced by LPS in N9 microglial cells in a concentration-dependent manner. Conclusions Compounds 7,9, and 10 are isolated from the Lauraceae for the first time. Compound 3 was moderate inhibition activities.
出处 《沈阳药科大学学报》 CAS CSCD 北大核心 2016年第1期14-19,共6页 Journal of Shenyang Pharmaceutical University
关键词 桂枝 神经保护 化学成分 结构鉴定 Cinnamomum cassia Presl ( Lauraceae ) neuroprotection chemical constituent structureidentification
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