摘要
建立了分子印迹固相萃取/高效液相色谱法测定猪肝中盐酸克伦特罗残留量的方法。以ASB C18色谱柱（250 mm×4.60 mm,5μm）分离,流动相为甲醇-水（45∶55,p H 3.0）,紫外检测波长243 nm,进样量10μL,流速0.6 m L/min,柱温30℃。分子印迹固相萃取条件为：上样溶剂为双蒸水,上样流速为0.6 m L/min;洗脱剂为1%三氟乙酸的甲醇溶液。上述优化条件下,该方法在0.432~4.32μg/m L范围内线性关系良好,相关系数（r）为0.999 0。加标回收率为84.7%~92.0%,相对标准偏差（RSD）为1.5%~1.8%。方法的准确度和精密度满足残留分析的要求。
A molecularly imprinted solid phase extraction coupled with high performance liquid chro- matographic （HPLC） method was firstly developed for the determination of clenbuterol hydrochloride residues in pork liver. The chromatographic conditions were as follows： the column： ASB C18 col- umn（250mm x 4. 60 mm, 5 μm）, mobile phase： methanol-water （45 ： 55, pH3.0）, UV detection wavelength： 243 nm, sample volume： 10μL, flow rate： 0. 6 mL/min, column tempera- ture ： 30 ℃. Molecularly imprinted solid phase extraction conditions were as follows ： solvent of sam- ple loading： double distilled water, flow rate of loading： 0. 6 mL/min; elution solvent： methanol solution containing 1% trifluoroacetic acid. Under the optimal conditions, the calibration curve was linear in the range of 0. 432 - 4.32 μg/mL, with linear equation of y = 30 358x - 307.74 （ r = 0. 999 0）. The spiked recoveries were in the range of 84.7% - 92. 0% , with RSDs of 1.5% - 1.8%. The accurancy and precision of the method could satisfy the requiremennts for residuals analysis.
出处
《分析测试学报》
CAS
CSCD
北大核心
2016年第1期115-118,共4页
Journal of Instrumental Analysis
基金
江西省科技厅支撑项目(2010BSA17500)
关键词
分子印迹固相萃取
高效液相色谱法
猪肝
盐酸克伦特罗
molecularly imprinted solid phase extraction
high performance liquid chromatography（HPLC）
pork liver
clenbuterol hydrochloride
