摘要
目的观察芩丹颗粒减轻高温暴露大鼠热损伤的作用,并探讨其机制。方法将75只SD大鼠随机分为常温对照组、热损伤模型组和芩丹颗粒低、中、高剂量组,每组各15只;热暴露前48 h芩丹颗粒低、中、高剂量组分别给予生理盐水、生理盐水及生理盐水+芩丹颗粒10、20、40 g/kg灌胃,常温对照组、热损伤模型组均给予等量生理盐水灌胃。将热损伤模型组和芩丹颗粒组暴露于43℃热仓中,持续75 min,实时监测体核温度(Tc)和平均动脉压(MAP)变化,热损伤后迅速取出;常温对照组置于室温环境中。检测各组大鼠血清谷草转氨酶(AST)、谷丙转氨酶(ALT)、尿素氮(BUN)、肌酐(Cr)、白介素1β(IL-1β)及肿瘤坏死因子α(TNF-α)水平。结果热损伤模型组Tc、AST、ALT、SUN、Cr、IL-1β及TNF-α水平均高于常温对照组,MAP低于常温对照组(P均<0.05);与热损伤模型组比较,芩丹颗粒高剂量组Tc、AST、ALT、SUN、Cr、IL-1β及TNF-α水平下降,MAP升高(P均<0.05)。结论芩丹颗粒可减轻高温暴露大鼠热损伤,其机制可能与减轻炎症反应有关。
Objective To investigate the function of Qindan particles in reducing heat-induced injury of rats exposed to high temperature and its mechanism. Methods Seventy-five SD rats were randomly divided into the normothermic control group,heat injury model group,low-dose Qindan group,medium-dose Qindan group and high-dose Qindan group,15 rats in each group. Rats in the heat injury model and Qindan groups were orally administered saline or Qindan 10,20 and40 g / kg,respectively,followed by exposure to heat（ 43 ℃ for 75 min） before recovery at room temperature（ RT,24 ℃）.Meanwhile,the body temperature（ Tc） and mean arterial pressure（ MAP） was monitored. After the thermal damage,the blood was collected immediately to detect the serum levels of aspartate aminotransferase（ AST）,alanine aminotransferase（ ALT）,blood urea nitrogen（ BUN）,creatinine（ Cr）,interleukin-1β（ IL-1β） and tumor necrosis factor-α（ TNF-α）.Results Compared with the normothermic control group,the levels of Tc,AST,ALT,SUN,Cr,IL-1β and TNF-α were all higher,but the MAP was lower in the heat injury model group（ all P〈0. 05）. Compared with the heat injury model group,the levels of Tc,AST,ALT,SUN,Cr,IL-1β and TNF-α were all lower but the MAP was higher in the high-dose Qindan group（ all P〈0. 05）. Conclusion Qindan particles attenuate heat-induced multiple organ injury,whose mechanism may be related with reducing inflammation in rats.
出处
《山东医药》
CAS
北大核心
2016年第2期11-13,共3页
Shandong Medical Journal
基金
国家自然科学基金资助项目(31071042)
吉林省卫生厅科学技术研究项目(2012Z066)
关键词
芩丹颗粒
高温暴露
谷草转氨酶
谷丙转氨酶
尿素氮
肌酐
白细胞介素
肿瘤坏死因子Α
大鼠
Qindan particles
high temperature exposure
aspartate aminotransferase
alanine aminotransferase
urea nitrogen
creatinine
interleukin
tumor necrosis factor-α
rats
