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全反式维甲酸联合LY294002对食管癌EC1细胞增殖、迁移及PI3K、MMP-2表达的影响 被引量:5

Effect of all-trans retinoic acid combined with LY294002 on proliferation,migration and expressions of PI3K and MMP-2 in EC1cells
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摘要 目的:探讨全反式维甲酸(ATRA)、磷脂酰肌醇激酶-3(PI3K)抑制剂LY294002单独或联合作用对食管癌EC1细胞增殖、迁移及PI3K和MMP-2表达的影响。方法:将常规培养的EC1细胞随机分为ATRA组、LY294002组、ATRA+LY294002组和对照组4组,采用CCK-8检测各组细胞的增殖情况,通过划痕实验测定各组细胞的迁移能力,采用RT-PCR和Western blot方法检测各组细胞中PI3K及MMP-3 mRNA和蛋白的表达情况。结果:作用24、48和72 h后,ATRA+LY294002组的细胞增殖抑制率均高于其他2组(P<0.05);作用24 h后,ATRA+LY294002组细胞迁移能力均低于其他3组(P<0.05);作用48 h后,ATRA+LY294002组细胞PI3K及MMP-2 mRNA和蛋白的表达量均低于其他3组(P<0.05)。结论:ATRA联合LY294002可协同抑制EC1细胞的增殖、迁移和PI3K、MMP-2的表达,进而可能协同抑制肿瘤血管生成。 Aim: To investigate effects of all-trans retinoic acid( ATRA),LY294002 alone or combined on proliferation,migration and phosphoinositide 3-kinase( PI3K),matrix metalloproteinase-2( MMP-2) expressions of EC1 cells in vitro. Methods: The cultured EC1 cells were randomly allocated into four groups: ATRA group,LY294002 group,ATRA plus LY294002 group and control group. The proliferation rate was detected by CCK-8 assay. Wound healing assay was used to observe the migration rate. The mRNA and protein expressions of PI3 K and MMP-2 in the cells were detected by RT-PCR and Western blot assay. Results: The cell proliferation inhibition rate of ATRA plus LY294002 was higher than those of the other three groups after 24,48,and 72 h treatment( P〈0. 05). The migration rate of ATRA plus LY294002 group was lower than those of the other three groups after 24 h treatemnt( P〈0. 05). The relative expressions of PI3 K and MMP-2 mRNA and protein of ATRA plus LY294002 group were lower than those of the other three groups after 48 h treatment( P〈0. 05). Conclusion: ATRA combined with LY294002 could inhibit EC1 cells' proliferation,migration and the expressions of PI3 K and MMP-2,which may be related to the inhibition of tumor angiogenesis.
出处 《郑州大学学报(医学版)》 CAS 北大核心 2016年第1期1-5,共5页 Journal of Zhengzhou University(Medical Sciences)
基金 河南省科技攻关计划项目112102310154
关键词 全反式维甲酸 LY294002 EC1细胞 PI3K MMP-2 all-trans retinoic acid LY294002 EC1 cell phosphoinositide 3-kinase matrix metalloproteinase-2
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