摘要
目的:探讨沉默结肠癌转移相关基因1(MACC1)对卵巢癌上皮性癌细胞SKOV-3顺铂化疗敏感性的影响。方法:SKOV-3细胞分为shRNA转染组(重组质粒psuper-EGFP-s3转染)、空质粒转染组(psuper-EGFP-neo转染)和对照组(未作转染),采用RT-PCR法和Western blot法检测各组细胞MACC1 mRNA和蛋白的表达,MTT法检测细胞对顺铂的耐药性。结果:3组SKOV-3细胞MACC1 mRNA和蛋白表达比较,差异有统计学意义(P<0.05),shRNA转染组较未转染组和空质粒组下降。shRNA转染组对顺铂的IC50较对照组和空质粒转染组下降(P<0.05)。结论:沉默MACC1的表达能增加卵巢癌SKOV-3细胞对顺铂的敏感性。
Aim： To investigate the relationship of RNA interfering MACC1 and cisplatin sensibility of ovarian cancer cell line SKOV-3. Methods： SKOV-3 cells were divided into 3 groups： shRNA group（ recombinant plasmid psuper-EGFPs3 transfected SKOV-3 cells）,empty plasmid transfection group（ psuper-EGFP-neo transfected SKOV-3 cells） and control group（ non transfected SKOV-3 cells）. RT-PCR and Western blot were used to detect the expressions of MACC1 mRNA and protein. IC50 of cisplatin in different groups was determined by MTT assay. Results： The expressions of MACC1 mRNA and protein in the 3 groups were significantly different（ P〈0. 05）,and those of the shRNA transfection group were lower than those in the control group and empty plasmid group. The shRNA transfection group had lower IC50 than the control group and empty plasmid group（ P〈0. 05）. Conclusion： The sensitivity of SKOV-3 cells to cisplatin could be enhanced by RNA interfering MACC1 gene.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2016年第1期92-95,共4页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省重点科技攻关计划项目122102310545
