摘要
目的:观察激光针刀配合穴位注射威灵仙总皂苷对膝骨关节炎模型大鼠血清类风湿因子(RF)及前列腺素E_2(PGE_2)的影响,探讨其作用机制。方法:大鼠60只,随机分为空白对照组、模型组、激光针刀组、穴位注射组及综合治疗组,除空白对照组外其它4组动物于左前侧膝关节腔内注射弗氏完全佐剂(0.1 ml)造模。在造模成功后,模型组腹腔注射生理盐水;激光针刀组取左前肢循经取穴为主穴激光针刀治疗,辅以电针刺激关元、足三里、脾俞、气海、阳陵泉、肾俞、肝俞等配穴20 min;穴位注射组于左前侧膝骨关节腔内每日穴位注射威灵仙总皂苷(50 mg/kg);综合治疗组治疗方法为穴位注射组加综合治疗组。以上各组均连续治疗14天。测定动物关节腔积液中透明质酸(HA)、白介素IL-6、前列腺素E_2(PGE_2)、血清类风湿因子(RF)Ig M-RF、Ig A-RF阳性率,病理检查关节软骨MMP-1细胞阳性表达率。结果:激光针刀配合穴位注射威灵仙总皂苷显著降低模型大鼠IL-6、PGE_2、Ig M-RF、IgA-RF及MMP-1等指标,升高HA,与本组治疗前、模型组、激光针刀组及穴位注射组比较(P<0.05),差异有统计学意义。结论:激光针刀配合穴位注射威灵仙总皂苷能有效治疗实验性大鼠膝骨关节炎,其作用机制可能为激光针刀松解膝关节粘连的炎症组织,电针刺激激发经气,威灵仙总皂苷降低IL-6及PGE_2等炎性因子刺激,提高关节腔内HA含量,阻断类风湿因子与抗原IgM、IgA结合,降低IgM-RF、IgA-RF合成,减轻变态反应发生而起到协同治疗作用。
Objective： Establishing the experimental operation for model rats with knee osteoarthritis, to observe the effect of laser needle knife combined with acupoint injection of Radix Clematidis saponins on serum rheuma- toid factor（RF） RA and PGE2, and to investigate its mechanism of action. Methods.60 rats were randomly di- vided into a blank control group, a model group, a laser acupuncture group, an acupoint injection group and a combined treatment group, except the blank control group in other four groups animals received the left knee joint cavity injection of Freundg complete adjuvant （0.1 ml）. After the success of modeling,the model group was injected with saline; laser needle knife group received eleetroacupuncture combined with laser needle knife treatment, electroacupuncturing at Guanyuan, Zusanli, Pishu, Qihai, Yanglingquan, Shenshu, Ganshu for 20 min ;the acupoint injection group received the left knee joint cavity acupoint injection（50mg/kg） ; comprehen- sive treatment method was used for acupoint injection group and combined treatment group; each group was treated for 14 days. Determine animal articular effusion of hyaluronic acid （HA） and interleukin 6 IL - and prostaglandin E2 （ PGE2） , serum rheumatoid factor （ rheumatoid, factor, RF） IgM - RF, IgA - RF positive rate, the positive expression rate of MMP - 1 cells in pathological articular cartilage. Results：The laser knife combined with acupoint injection of Radix Clematidis saponins could significantly decrease the IL - 6, PGE2, IgM - RF, IgA - RF and could MMP - 1 and increase HA. Compared with the model group, the laser needle knife group and acupoint injection group had a significant difference（ P 〈 0.05 ）. Conclusion： Laser knife com- bined with acupoint injection of Radix Clematidis saponins can be effective in the treatment of rats with knee os- teoarthritis, and its mechanism may be related to the laser acupotomy knee joint adhesion in inflammation tissue, electroacupuncture stimulation, reduction of IL -6 and PGE2and other inflammatory factors stimulation, increase of HA content in articular cavity. Prevent rheumatoid factor from binding with antigen IgM and IgA, and de- crease the combination of IgM - RF and IgA - RF, reduce the occurrence of allergic reactions and play a syner-gistic effect.
出处
《针灸临床杂志》
2015年第12期71-74,共4页
Journal of Clinical Acupuncture and Moxibustion
关键词
激光针刀
威灵仙总皂苷
膝骨关节炎
前列腺素E2
类风湿因子
穴位注射
Laser needle knife
Total saponins of Radix Clematidis
Knee osteoarthritis
PGE2
Rheumatoidfactors
Acupoint injection
