摘要
目的:通过体征、肾功能、神经内分泌激素、血清电解质、肾脏组织形态等环节,探讨参附强心丸对慢性肾衰竭大鼠温阳利水的作用与机制。方法:采用"单肾切除合并腺嘌呤灌胃法"制备大鼠CRF模型,按随机数字表法分为模型对照组、地塞米松对照组、参附强心丸2.14、1.07、0.54 g生药/kg剂量组,观察各组大鼠神经内分泌激素、肾功能、血电解质、体温、肾脏质量与肾体比、肾脏的组织形态等指标。结果:模型对照组大鼠出现皮毛稀疏,蜷卧少动,消瘦等衰弱体征,参附强心丸3个剂量组大鼠一般状况较模型对照组明显好转;模型对照组大鼠尿素氮(UREA)、肌酐(CREA)含量明显升高,参附强心丸3个剂量组和地塞米松对照组UREA、CREA水平均明显低于模型对照组;模型对照组和参附强心组大鼠的血管紧张素Ⅱ(Ang II)显著低于假手术对照组,醛固酮(ALD)明显高于假手术对照组,参附强心丸2.14 g/kg和地塞米松对照组大鼠的ALD较模型对照组明显降低,差异均有统计学意义(P<0.05)。模型对照组大鼠呈高钾、低钙、高磷血症,参附强心丸2.14 g/kg组大鼠可纠正低血钙症,3个剂量组均能纠正高磷血症,地塞米松对照组可纠正大鼠高钾、低钙、高磷血症,差异均有统计学意义(P<0.05)。模型对照组大鼠体温明显降低,地塞米松组体温过低;模型对照组肾脏质量明显大于假手术对照组,参附强心丸2.14 g/kg组大鼠肾脏质量较模型对照组降低,且肾体比明显低于模型对照组,差异均有统计学意义(P<0.05)。肉眼观察及肾脏病理学显示,参附强心丸3个剂量组及地塞米松组大鼠肾脏大小及病变有不同程度减轻。结论:参附强心丸对大鼠肾阳虚有改善作用,可保护肾功能、改善水钠潴留状态、纠正电解质紊乱、可使病变的肾脏组织结构得以修复。
OBJECTIVE： To probe into the effects and mechanism of Shenfuqiangxin pills on the warming yang for diuresis of chronic renal failure rats via the link of signs,renal function,neuroendocrine hormone,serum electrolytes and renal tissue morphology,etc. METHODS： The CRF model of rat was established by ＂unilateral nephrectomy combined with gavageing adenine ＂,the rats were divided into the model control,dexamethasone group and the dosage group with Shenfuqiangxin pills of 2. 14 g / kg,1. 07 g / kg,0. 54 g / kg in crude drug via the random number table. The indexes of neuroendocrine hormone,renal function,blood electrolytes,body temperature,ratio of the kidney weight versus kidney volume and the form of organization of kidney in rates were observed. RESULTS： Some asthenia signs like sparse fur,sedentary lying and emaciation were observed in rats in mode group. The general conditions in three dosage groups of Shenfuqiangxin pills had been significantly improved. Level of UREA and CREA contents increased significantly in mode group,which in three dosage groups of Shenfuqiangxin pills were significantly lower than that in mode group. In mode group and three dosage groups of Shenfuqiangxin pills,angiotensin Ⅱ（ AngⅡ） were significantly lower than that of sham-operation group,but aldosterone（ ALD） were significantly higher than that of sham-operation group. Compared with the mode group,ALD in dosage group with 2. 14 g / kg Shenfuqiangxin pills and dexamethasone group were obviously decreased,thedifferences were statistically significant（ P〈0. 05）. Hyperkalemia,low calcium and hyperphosphatemia of rats were observed in the mode group,dosage group with 2. 14 g / kg Shenfuqiangxin pills could correct hypocalcemia,and three dosage groups of Shenfuqiangxin pills all could correct hyperphosphatemia; the dexamethasone group could correct hyperkalemia,low calcium and hyperphosphatemia of rats,the differences were statistically significant（ P〈0. 05）. The body temperature of rats in mode group were significantly decreased,but hypothermia were observed in dexamethasone group. The kidney weight in mode group were significantly higher than that in sham-operation group; compared with the mode group,kidney weight in dosage group with 2. 14 g / kg Shenfuqiangxin pills decreased,and the kidney volume was smaller,the difference was statistically significant（ P〈0. 05）. From the visual inspection and renal pathology,it indicated that the kidney size and lesions both had various degrees of relief in three dosage groups of Shenfuqiangxin pills and dexamethasone group. CONCLUSIONS： Shenfuqiangxin pills can improve the function of kidney-yang deficiency rats,protect the renal function,improve water and sodium retention,correct the electrolyte imbalance,and repair the renal lesions.
出处
《中国医院用药评价与分析》
2015年第10期1309-1312,共4页
Evaluation and Analysis of Drug-use in Hospitals of China
基金
国家自然科学基金青年基金项目(G81202801)
关键词
参附强心丸
腺嘌呤
慢性肾衰和肾阳虚大鼠
温阳利水
血管紧张素Ⅱ
Shenfuqiangxin pills
Adenine
Chronic renal failure and kidney-yang deficiency rats
Warming yang for diuresis
Angiotensin Ⅱ
