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利用代谢工程构建高产乙偶姻枯草芽孢杆菌 被引量:2

Metabolic engineering of acetoin high producing Bacillus subtilis
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摘要 乙偶姻是枯草芽孢杆菌的主要胞外产物,在代谢工程育种中,阻断目标产物的降解途径或加强目标产物前体物的代谢通量是有效提高目的产物积累量的重要手段。该研究克隆了来自枯草芽孢杆菌JNA 3-10的α-乙酰乳酸合成酶(ALS)和α-乙酰乳酸脱羧酶(ALDC)基因als S和als D。在大肠杆菌BL21中分别对ALS和ALDC进行表达、纯化和初步的酶学性质研究,随后将ALS、ALDC、ALS+ALDC分别在乙偶姻还原酶敲除菌株(BM)中进行过量表达,发现3株重组菌株的乙偶姻产量与BM菌株相比分别提高了5.2%、4.1%和10.8%,最终乙偶姻产量达到53.5 g/L,生产效率为0.50 g/(L·h)。 Bacillus subtilis produces acetoin as a major extracellular product. In metabolic engineering breeding,blocking product degradation pathway or enhancing precursor metabolism flux is the important way to improve the accumulation of the product. For genetic engineering breeding of an acetoin high production B. subtilis,the key enzymes α-acetolactate synthetase( ALS) and α-acetolacetate decearboxylase( ALDC) on acetoin synthesis pathway were overexpressed. Compared with BM strain,the overexpression of ALS,ALDC and ALS + ALDC increased acetoin probuction by5. 2%,4. 1% and 10. 8%,respectively,but the biomass was inhibited by overexpression of these enzymes. With a long fermentation duration,the highest acetoin production and productivity was 53. 5 g / L and 0. 50 g /( L·h).
出处 《食品与发酵工业》 CAS CSCD 北大核心 2015年第12期18-25,共8页 Food and Fermentation Industries
基金 国家自然科学基金(31500065,21276110,31400082) 江苏省青年自然科学基金(BK20150142) 中央高校基本科研业务费专项资金(JUSRP11544)
关键词 乙偶姻 枯草芽孢杆菌 代谢工程 α-乙酰乳酸合成酶 Α-乙酰乳酸脱羧酶 acetoin Bacillus subtilis metabolic engineering α-acetolactate synthetase α-acetolacetate decearboxylase
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参考文献20

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