摘要
为研究椒江化工园区邻近海域沉积物中细菌结构,选取四个典型站点,通过分离纯化、鉴定和建立克隆文库的方法,对其进行多样性及系统发育分析。可培养细菌的形态学及API鉴定结果显示洋葱假单胞菌及杀鲑气单胞菌杀鲑亚种是优势种,典型细菌16S r DNA分子鉴定结果表明厚壁菌门及γ-变形菌纲为主要类群。非培养细菌克隆文库的序列分析结果表明:细菌主要包括变形菌门、酸杆菌门、绿弯菌门、芽单胞菌门、硝化螺旋菌门、CFB群、放线菌门、厚壁菌门、浮霉菌门等9个类群;其中C3站点克隆子主要属于γ-变形菌纲及δ-变形菌纲;C4站点克隆子以γ-变形菌纲及酸杆菌门为主;C5站点克隆子主要属于δ-变形菌纲及γ-变形菌纲;C6站点克隆子主要属于γ-变形菌纲及放线菌门。综合可培养及非培养结果,可发现椒江口化工园区邻近海域沉积物中γ-变形菌纲为典型优势类群,除相当数量的克隆子相似序列来自石油烃污染的沉积环境外,还有大量克隆子相似序列来自养殖污染环境。
We studied the community structure of bacteria in sediment from four sites in Jiaojiang chemical industrial park adjacent area and phylogenesis in bacterial clone library by isolation, purification, and identification procedures. The results of 16 S r DNA analysis showed that for cultured bacteria, Aeromonas salmonicida was the main species, and Firmicutes and γ-Proteobacteria were the dominant groups; while non-cultured bacteria included mainly Proteobacteria, Acidobacteria, Chloroflexi, Gemmatimonadetes, Nitrospira, CFB group, Actinobacteria, Firmicutes and Planctomycetes. Each site featured own bacterial clone. In overall, γ-Proteobacteriain was the dominant group in sediment of Jiaojiang chemical industrial park adjacent area, and the similar sequences of many typical clones were probably derived from environment pollution by petroleum hydrocarbon, aquaculture and heavy metals.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2015年第6期1420-1437,共18页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金面上项目
31270160号
浙江省自然科学基金项目
LY12C03003号
浙江省环保厅科研项目
2012A033号
2013A020号
