摘要
为建立高效高抗性淀粉(RS)水稻新品种培育的分子标记辅助选择育种体系,以降糖稻1号为高RS基因供体材料,分别与产量较高的密阳23、秀水123和沪稻55进行杂交,并利用3个杂交群体早世代籽粒垩白特性、直链淀粉含量(AC)及功能标记(CAPS/Spe I)进行高RS水稻辅助选择育种。结果表明,籽粒的垩白特性与RS含量有很高的关联性,早世代通过垩白度和垩白粒率可以淘汰大部分低RS含量单株。利用控制水稻RS合成主效基因sbe3-rs上的一个功能标记(CAPS/Spe I)确定sbe3-rs基因型纯合的高RS单株,经连续自交和辅助选育,最终培育了3个兼具功能性、高产和优质的高RS水稻品系RS23、RS123和RS55。本研究首次提出利用对杂交群体早世代籽粒垩白特性挑选淘汰大部分低RS含量单株,同时结合分子标记辅助选择显著提高选择效率和准确度,建立了高RS水稻品种选育的高效分子标记辅助选择育种体系。本研究可为开展高RS育种提供新的理论依据和基础材料。
For establishment of marker-assisted selection（ MAS） system for breeding rice varieties with high resistant starch content,Jiangtangdao 1 was used as a high RS donor parent and crossed with three high yield rice varieties Miyang23,Xiushui123 and Hudao55 respectively. Grain chalkiness,amylose content（ AC） and functional marker（ CAPS /Spe I） we used as assistant markers for high RS content selection in the early generation. The results showed that grain chalkiness characteristics were positively correlated with RS content. Most of low RS content plants could be eliminated according to chalkiness degree and high chalky grain percentage. The high RS homozygous plants could be precisely identified by further utilization of a functional marker CAPS / Spe I of sbe3- rs. Through continuous selffertilization and marker-assisted selection（ MAS）,three rice varieties RS23,RS123 and RS55 with high yield,good quality and high RS content were bred. In conclusion,it is feasible to efficiently eliminate most of low RS-content plants in early generations by choosing seeds with high chalkiness characteristics,moreover,the combination of utilization a functional marker could significantly improve the selection efficiency and accuracy. In this study,an efficient MAS system for breeding of high RS content rice varieties was established which paved the way for providing new theoretical basis and materials for RS breeding in the future.
出处
《核农学报》
CAS
CSCD
北大核心
2015年第12期2259-2267,共9页
Journal of Nuclear Agricultural Sciences
基金
上海市基础研究重点项目(13JC1408600)
上海市自然科学基金(13ZR1460800)
2015年度上海市农业科学院青年人才成长计划(沪农青字(2015)第1-1号)
中韩国际合作项目(PJ01125703)
韩国农村振兴厅agenda项目(PJ010086)
韩国农村振兴厅未来生物环保项目(PJ011257)
关键词
水稻
抗性淀粉
垩白粒率
垩白度
分子标记辅助选择
rice
resistant starch
chalky grain percentage
chalkiness degree
marker-assisted selection
