摘要
目的探讨沉默HepG2细胞株中MALAT1基因对蜂毒素诱导的细胞增殖抑制和凋亡的影响。方法采用MTT法检测蜂毒素对HepG2细胞的增殖抑制作用;流式细胞仪检测细胞凋亡率;qPCR法检测HepG2细胞中MALAT1基因的表达;采用特异性siRNA对HepG2细胞的MALAT1基因进行沉默;比较单独用蜂毒素处理和给予蜂毒素同时沉默MALAT1的细胞增殖抑制率和凋亡率变化。结果蜂毒素明显抑制HepG2细胞的增殖并促进细胞凋亡,呈浓度依赖性;和正常肝细胞株L0-2相比,MALAT1 mRNA在HepG2细胞中存在高表达(P<0.05);蜂毒素可下调细胞中MALAT1的表达,并随着浓度的增加抑制率升高;给予蜂毒素同时沉默MALAT1的研究组中,细胞增殖抑制和凋亡率都明显高于单独给予蜂毒素组(P<0.05)。结论蜂毒素可下调HepG2细胞株中MALAT1的表达,且沉默MALAT1可促进蜂毒素诱导的HepG2细胞增殖抑制和凋亡。
Aim To investigate the effects of silencing MALATl gene on cell proliferation inhibition and apoptosis induced by Melittin in human hepatocellular carcinoma HepG2 cells.Methods The inhibitory rate of cell proliferation treated with Melittin in HepG2 cells was examined by MTT assay.Apoptotic rate was detected by flow cytometry.The MALAT1 expression level in HepG2 cells was measured by qPCR.Specific siRNAs were utilized to silence MALAT1 expression.The rates of cell proliferation inhibition and apoptosis in HepG2 cells treated with siRNA and Melittin were compared with those of Melittin alone.Results Melittin significantly suppressed the growth of HepG2 and induced cell apoptosis in a dose-dependent manner.Compared with normal liver cell lines,MALAT1 was highly expressed in HepG2 cells(P < 0.05).The expression of MALAT1 in HepG2 cells was inhibited by Melittin,and the inhibitory rate increased with the increase of concentration.The rates of cell proliferation inhibition and apoptosis in HepG2 cells treated with siRNA and Melittin were significantly higher than those treated merely with Melittin.Conclusion Melittin can reduce the expression of MALAT1 and silencing MALAT1 can effectively promote proliferation inhibition and apoptosis in HepG2 cells induced by Melittin.
出处
《中国药理学通报》
CAS
CSCD
北大核心
2016年第2期211-216,共6页
Chinese Pharmacological Bulletin
基金
国家自然科学基金资助项目(No 81473268
81273526)
高校博士点基金资助项目(No 20123420120001)
安徽省自然科学基金资助项目(No 1408085MKL31
1308085MH145)
