摘要
为研究氮源、氮浓度及磷浓度对一株富油海水藻株Desmodesmus sp.WC08生长和油脂积累的影响。以BG11为基本培养基,考察亚硝酸钠、硝酸钠、尿素及氯化铵以及不同尿素浓度和磷浓度对Desmodesmus sp.WC08生长、油脂积累影响。结果表明:除氯化铵外,不同氮源对Desmodesmus sp.WC08生长影响较小,以尿素为氮源时,生长和油脂积累最佳;Desmodesmus sp.WC08生长和油脂积累的最佳氮浓度为10 mmol/L,过高或过低都将影响其生长和油脂积累;Desmodesmus sp.WC08的生长和油脂积累随着磷浓度的升高先增加后降低,最佳磷浓度为0.5 mmol/L;在最佳氮浓度和磷浓度下,Desmodesmus sp.WC08对氮、磷的吸收较完全,能够充分利用培养基中的营养物质。
In order to study the effects of nitrogen sources and the concentrations of nitrogen and phosphorus on the growth,lipid accumulation of a marine algae Desmodesmus sp. WC08. Four nitrogen sources such as sodium nitrite,sodium nitrate,urea and ammonium chloride and different concentrations of urea and phosphorus were investigated based on the BG11 media. The results showed that different nitrogen sources made little difference to Desmodesmus sp. WC08 except for ammonium chloride. In addition,the growth and lipid accumulation proved to be best when using urea as nitrogen source. Accordingly,the best growth and lipid accumulation which would be easily affected if the concentration of nitrogen was higher or lower,could be obtained at the optimal concentration of nitrogen（10 mmol/L）. Likewise,the growth and lipid accumulation could be obtained at the optimal concentration of phosphorus（0.5 mmol/L）. To be concluded,the nitrogen and phosphorus could be thoroughly absorbed by WC08 and the nutrients in the BG11 media could also be fully used at the their optimal concentrations.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第4期223-227,共5页
Science and Technology of Food Industry
基金
国家科技支撑计划项目课题(2011BAD14B01)
海南省中药现代化科技专项(ZY201327)
国家科技型中小企业技术创新基金(13C26244604892)
海南省产学一体化项目(CXY20150034)
