摘要
目的探讨皮质醇增多症肾上腺皮质不同病变中脆性组氨酸三联体(FHIT)、Ki-67及增殖细胞核抗原(PCNA)的表达水平及其在鉴别诊断中的作用。方法应用免疫组织化学方法检测49例皮质醇增多症肾上腺皮质病变(腺癌14例、腺瘤26例、皮质增生9例)患者FHIT、Ki-67及PCNA的表达。结果 FHIT在肾上腺皮质增生表达最高,腺瘤次之,腺癌最低(分别为100.00%、96.15%、42.86%);Ki-67与PCNA在肾上腺皮质腺癌的表达最高,腺瘤次之,皮质增生最低(分别为85.71%、7.69%、0和100.00%、96.15%、77.78%)。FHIT、Ki-67及PCNA在皮质醇增多症肾上腺皮质病变中的表达两两相关,其中FHIT与Ki-67表达呈负相关(r=-0.718,P=0.00),FHIT与PCNA表达呈负相关(r=-0.449,P=0.001),Ki-67与PCNA表达呈正相关(r=0.387,P=0.006)。联合检测FHIT、Ki67及PCNA结果显示,腺癌表现为FHIT阴性或弱阳性、Ki-67强阳性、PCNA强阳性;腺瘤表现为FHIT阳性、Ki-67阳性、PCNA阳性;皮质增生表现为FHIT强阳性、Ki-67阴性、PCNA阴性。结论联合检测FHIT、Ki-67及PCNA对肾上腺皮质腺癌、腺瘤及增生的鉴别诊断具有重要的价值。
Objective To study the expression of fragile histindine triad（FHIT）,Ki-67 and proliferation cell nuclear antigen（PCNA）among different adrenocortical diseases in hypercortisolism and their role in the differential diagnosis.Methods The expressions of FHIT,Ki-67 and PCNA were detected by immunohistochemical staining in 49 cases of adrenocortical diseases,including14 cases of adrenocortical adenocarcinoma,26 cases of adrenocortical adenoma and 9cases of adrenocortical hyperplasia.ResultsThe expression rate of FHIT in adrenocortical hyperplasia was highest,followed by adrenocortical adenoma and which in adrenocortical adenocarcinoma was lowest（100.00%,96.15% and 42.86%）.The expression rate of Ki-67 in adrenocortical adenocarcinoma and adrenocortical adenoma was 85.71% and 7.69%respectively,while there was no expression at all in adrenocortical hyperplasia（0）.The expression rate of PCNA in adrenocortical adenocarcinoma,adrenocortical adenoma and adrenocortical hyperplasia was100.00%,96.15% and 77.78%,respectively.The expression of FHIT,Ki-67 and PCNA in adrenocortical diseases of hypercortisolism was pairwise correlated,in which the FHIT expression was negatively correlated with the Ki-67expression（r=-0.718,P=0.00）and the FHIT expression was negatively correlated with the PCNA expression（r=-0.449,P=0.001）,while the Ki-67 expression was positively correlated with the PCNA expression（r=0.387,P=0.006）.The combined detect of FHIT,Ki-67 and PCNA expressions indicated that adrenocortical adenocarcinoma showed negative or weak positive FHIT,strongly positive Ki-67 and PCNA;adrenocortical adenoma was manifested by positive FHIT,Ki-67 and PCNA;adrenocortical hyperplasia was manifested by strong positive FHIT,negative Ki-67 and PCNA.Conclusion The combined detection of FHIT,Ki-67 and PCNA has an important value for the differential diagnosis of adrenocortical adenocarcinoma,adrenocortical adenoma and adrenocortical hyperplasia.
出处
《重庆医学》
CAS
北大核心
2016年第5期580-583,587,共5页
Chongqing medicine
基金
国家自然科学基金资助项目(81060220)
广西自然科学基金资助项目(2011GXNSFA018172)
广西留学回国人员科学基金资助项目(桂科回0731019)
广西青年科学基金资助项目(桂科青0728073)
广西卫生厅自筹经费科研课题资助项目(Z2013027)
