摘要
目的建立高效液相-电雾式检测器(HPLC-CAD)方法测定远志药材中细叶远志皂苷的含量。方法色谱柱为Ultimate XB-C18柱(250mm×4.6mm,5μm);柱温25℃;流动相为甲醇-0.3mL·L^(-1)磷酸溶液(60︰40);流速1mL·min^(-1);进样量10μL;电雾式检测器,雾化温度25℃,采集频率10Hz。结果细叶远志皂苷峰面积与质量浓度线性回归方程:Y=4.295 4 X-0.135 8,r=0.999 9(n=6),线性范围为1.0~10.0mg·mL^(-1);平均回收率为101.4%。结论该方法高效、快速、准确,可作为药物及制剂中细叶远志皂苷的含量测定方法。
Objective To establish a high performance liquid chromatographic with charge aerosol detection（HPLC-CAD）method for the determination of tenuifolin in Radix polygalae.Methods Ultimate XB-C18column（250mm×4.6mm,5μm）was used in the study and the column temperature was 25 ℃,and methanol-0.3mL·L^-1 phosphoric acid（60︰ 40）solution as the mobile phase with a flow rate at 1mL·min^-1;the injection volume was 10μL;CAD：nebulization temperature was 25 ℃ and collection frequency was 10 Hz.Results The equation of linear regression was Y=4.295 4 X-0.135 8,r=0.999 9（n=6）,linear range was1.0-10.0mg·mL^-1,and the average recovery was 101.4%.Conclusion The method is accurate,rapid and high sensitive,which can be used in the quantitative determination of tenuifolin in protein pharmaceuticals.
出处
《西北药学杂志》
CAS
2016年第1期34-36,共3页
Northwest Pharmaceutical Journal
