摘要
目的：建立灭幽愈疡合剂中橙皮苷的含量测定方法。方法：采用HPLC法,色谱柱：BDS HYPERSIL-C18（4.6 mm×150 mm,5 sμm）;流动相：甲醇-0.1%磷酸溶液（38：62）;检测波长：283 nm;流速：0.8 m L/min;柱温：40℃。结果：橙皮苷在0.0413-0.8257μg之间,线性关系良好,加样回收率为99.65%,RSD（%）=0.11（n=6）。结论：该方法操作简便、准确,具有很好的重现性和稳定性,可以用于灭幽愈疡合剂的质量控制。
Objective： To select the method for Determination of hesperidin in Mieyou Yuyang oral solution by HPLC. Methods： The column employed was BDS HYPERSIL-C18（4.6 mm×150 mm, 5 sμm）;The mobile phase was methanol-0.1% phosphoric acid（38∶62）;The detection was effected at 283 nm; flow rate was 0.8 m L/min and column temperature was 40 ℃. Results： The linear range of hesperidin was 0.0413-0.8257 μg; the mean recovery was 99.65%, RSD=0.11%（n=6）. Conclusion： This method is simple,rapid and accurate with good reproducibility. It is suitable for the quality control of Mieyou Yuyang oral solution.
出处
《中医药导报》
2016年第1期46-48,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
