摘要
本试验采集了5只圈养成年大熊猫的新鲜粪便,用高通量测序技术,研究了大熊猫粪便中细菌和古菌的结构和组成。试验结果表明:圈养成年大熊猫粪便细菌主要由变形菌门Proteobacteria(74.45%)、厚壁菌门Firmicutes(15.66%)、拟杆菌门Bacteroidetes(4.34%)、蓝藻门Cyanophyta/色球藻纲Chroococcophyceae(4.01%)等组成,其中变形菌门主要以埃希氏杆菌属Esherichia/志贺氏菌属Shigella(49.84%)为主;厚壁菌门主要以梭菌属Clostridium(4.65%)为主;拟杆菌门主要以稳杆菌属Empedobacter(3.51%)为主;蓝藻门全部为未分类的色球藻纲(4.01%)。古菌主要由泉古菌门Crenarchaeota(55.99%)和广古菌门Euryarchaeota(42.33%)组成,其中优势古菌是热变形菌纲Thermoprotei(55.99%)中未分类的属和产甲烷菌属Methanogenium(24.70%)。
The structures and compositions of bacteria and archaeal in the feces of five adult captive giant pandas were studied using high-throughput sequencing technology. The results showed that the bacteria mainly consisted of Proteobacteria（ 74. 45% of total sequences）,Firmicutes（ 15. 66%）,Bacteroidetes（ 4. 34%）,and Cyanophyta/Chroococcophyceae（ 4. 01%） at the phylum level. Within the phylum Proteobacteria,Escherichia/Shigella（ 49. 84%） was the most abundant genus. Within the phylum Firmicutes,Clostridium（ 4. 65%） was the most abundant genus. Within the phylum Bacteroidetes,Empedobacter（ 3. 51%） was the most abundant genus. Within the phylum Cyanophyta / / Chroococcophyceae,only the unclassified Chroococcophyceae（ 4. 01%） was detected. The archaea mainly consisted of Crenarchaeota（ 55. 99%）and Euryarchaeota（ 42. 33%） at the phylum level,and the unclassified Thermoprotei（ 55. 99%） and Methanogenium（ 24. 70%） were the two dominant memberships at the genus level.
出处
《四川动物》
北大核心
2016年第1期17-23,共7页
Sichuan Journal of Zoology
基金
四川省科技厅国际合作项目(2013HH0043畜禽温室气体排放量检测及减排技术研究)
关键词
大熊猫
细菌
古菌
高通量测序
giant panda
bacterium
archaea
high-throughput sequencing
