摘要
采用急性染毒方法研究Cd^(2+)胁迫对不同发育阶段中华稻蝗胸部、腹部谷胱甘肽过氧化物酶(GPx)活性、谷胱甘肽硫转移酶(GST)活性和总抗氧化能力(T-AOC)的影响。结果表明,不同质量浓度的Cd^(2+)作用于不同发育阶段中华稻蝗体内时,其胸部和腹部的GPx,GST活性及T-AOC对Cd^(2+)胁迫的应激反应程度不同。说明Cd^(2+)可影响中华稻蝗的GPx,GST活性及T-AOC,中华稻蝗对Cd^(2+)有一定的耐受性,且这种耐受性与中华稻蝗的龄期及性别有关。中华稻蝗在受到Cd^(2+)胁迫时将调动其体内的抗氧化机制,维持H_2O_2产生与清除的平衡,有效地清除过量的ROS,保护机体免受外界不利因素的损害,进而避免机体DNA、蛋白质等功能的损伤。中华稻蝗可作为农业环境中Cd^(2+)的指示生物。
To explore and clarify the responses of Oxya chinensis to Cd^（2＋） stress, provide the scientific evaluation of Cd^（2＋） tolerance,the toxic effect of Cd^（2＋） on activities of glutathione peroxidase（GPx）and glutathione S-transferase（GST）, and the total anti-oxidative capacity（T-AOC）in different developing stage of O. chinensis injected with Cd^（2＋） were determined. Different developing stages O.chinensis were injected with Cd^（2＋） at different concentrations（0, 50, 100, 150, 200 mg/L）, and they were collected after 24 h. The results showed that the effect of Cd^（2＋） on GPx and GST activities and T-AOC were different within the experimental gradient. The GPx and GST activities, and T-AOC depended on the content of oxygen species（ROS）under Cd^（2＋） stress in the experiment, and Cd^（2＋） could induce damage of antioxidant defense system in O. chinensis, but O. chinensis kept its GPx and GST activities, and T-AOC increasing from Cd^（2＋） damage in some Cd^（2＋） contents. The tolerance of Cd^（2＋） changed in different developing stages and male or female O. chinensis. Variety of GPx and GST activities, and T-AOC in different developing stages and male or female O. chinensis might act as indicative functions for Cd^（2＋）contamination.
出处
《山西农业科学》
2016年第2期152-157,共6页
Journal of Shanxi Agricultural Sciences
基金
国家自然科学基金项目(31071980)
高等学校博士点基金项目(20111401110006)
山西省回国留学人员科研资助项目(2008114)
山西省农业科学院博士基金项目(YBSJJ0702)
关键词
中华稻蝗
镉
总抗氧化能力
谷胱甘肽过氧化物酶
谷胱甘肽硫转移酶
Oxya chinensis
cadmium（Cd）
total anti-oxidative capacity（T-AOC）
glutathione S-transferase（GST）
glutathione peroxidase（GPx）
