摘要
目的:研究烟草种子油的毒理学安全性。方法:参照卫生部《保健食品检验与评价技术规范》,采用急性经口毒性试验,检测烟草种子油有无急性毒性作用;采用Ames试验、小鼠骨髓细胞微核试验和小鼠骨髓细胞染色体畸变试验3项遗传毒性试验,检测烟草种子油有无致突变作用;采用30 d喂养试验检测烟草种子油亚慢性毒性。结果:小鼠14 d内未见中毒和死亡现象,即烟草种子油对小鼠的半数致死剂量(LD_(50))>21 500 mg/kg;Ames试验中烟草种子油各剂量组回变菌落数均未超过自发回变菌落数2倍,亦无剂量-效应关系,4株试验菌株,在加与不加S9时,均未呈现阳性结果;小鼠骨髓细胞微核试验中烟草种子油各剂量组小鼠的微核率在正常范围内,与阴性对照组相比,差异均无统计学意义(P均>0.05);小鼠骨髓细胞染色体畸变试验中阳性对照组染色体畸变率明显大于阴性对照组,烟草种子油各剂量组的染色体畸变率与阴性对照组间的差异均无统计学意义(P>0.05);30 d喂养结束后,未见试验动物有中毒及死亡状况,烟草种子油各剂量组的体质量、食物利用率、脏体比、血常规及血液生化指标与空白对照组相比均在正常范围内(P>0.05);病理组织学检查除烟草种子油高剂量组部分肝组织中见少量脂肪空泡外,各组大鼠主要脏器未见明显病理学改变。结论:在本实验条件下,烟草种子油未见明显的急性毒性,无致突变作用及亚慢性毒性作用。
OBJECTIVE：To evaluate the toxicity and safety of tobacco seed oil in mice.METHODS：Mice were orally exposed to tobacco seed oil for 14 and 30 days according to Care Food Inspection and Evaluation of Technical Specifications.Three genetic toxicology tests were used to evaluate mutagenic effects.In addition thirty day feeding test was conducted to detect sub-chronic toxicity.RESULTS：There was no evidence of toxicity or death in mice within 14 day,which indicated the median lethal dose（LD_（50） was more than 21 500 mg/kg.In the Ames test,the revertant colonies numbers in each dose group were two times less than the numbers of spontaneous revertant colonies,and no doseresponse relationship occurred.Four bacterial strains did not show positive results with or without S9 activation.In the mouse bone marrow cell micronucleus test,the rates from the exposed groups were similar to that of the negative control group（P〈0.05）.In the mouse bone marrow cell chromosome aberration test,the rates from the exposed groups were no significantly difference with the negative control group.After feeding for 30 days,there was no poisoning and death in the exposed mice.There was no significant difference from the control group in body weight,food utilization,organ-body ratios,and blood biochemical indexes.Histopathologic examination showed that fat vacuoles were observed in the liver tissue of high-dose group,but the other main organs of mice in the other groups had no pathological changes.CONCLUSION：Tobacco seed oil was non-toxic and did not show potentially mutagenic effects and sub-chronic toxicity within the text doses.
出处
《癌变．畸变．突变》
CAS
CSCD
2016年第1期60-65,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
十二五农村领域国家科技计划课题(2012BAD33B01-2)
