摘要
目的制备缓释定向释放型IgY微胶囊,并评价其效果。方法采用喷雾干燥法,以明胶、海藻酸钠为微胶囊壁材,制备抗仔猪病原性腹泻IgY微胶囊。以IgY的活性、包封率、体外释放性能及稳定性为评价指标,通过单因素分析和L_9（3_4）正交试验,分别从成囊材料的配比对微胶囊性能的影响、制备微胶囊成囊乳化液的稳定性影响因素以及喷雾干燥条件3方面对IgY微胶囊制备条件进行优化,并评价微胶囊的IgY活性、包封率、在人工胃液（simulated gastric fluid,SGF）和人工肠液（simulated intestinal fluid,SIF）中的缓释效果及稳定性。结果筛选出的制备微胶囊的最佳工艺条件为：明胶浓度4%、海藻酸钠浓度0.75%,按IgY与明胶-海藻酸钠质量比为3∶4加入IgY,乳化后按进风温度170℃,进料速度10 ml/min,干燥时间10 s,出口温度70℃进行喷雾干燥。在此工艺条件下制备的IgY微胶囊包封率为77.4%,活性保持在85%以上;在SGF中2 h释放率为8.6%,在SIF中4 h释放率为81.2%;90℃加热30 s仍可保持50%以上的活性,常温（25~30℃）放置10个月活性仍保持在90%以上,4℃保存10个月活性仅下降5%。结论本工艺制备的明胶-海藻酸钠IgY微胶囊具有生物活性高,稳定性强的特点,且对特异性IgY具有缓释和靶向作用。
Objective To prepare directional and sustained-released IgY microcapsules and evaluate its effect. Methods IgY mierocapsules against diarrhea in piglets were prepared by sparay drying technique using gelatin and sodium alginate as the wall materials. An L9（34） orthogonal experiment was performed to optimize the technological condition for preparation of the microeapsules based on the effect of formula of materials on the performance of microcapsules, the influencing factors of stability of encysted emulsion and condition for spray drying, serving the activity, encapsulation efficiency, in vitro release property and stability of IgY as the evaluation indexes. The microcapsules were evaluated for IgY activity, encapsulation efficiency as well as sustained-releases and stabilities in simulated gastric fluid （SGF） and simulated intestinal fluid （SIF）. Results The optimal technological condition for preparation of microcapsules was as follows： the concentrations of gelatin and sodium alginate were 4% and O. 75% respectively. IgY was mixed with gelatin-sodium alginate at a mass ratio of 3 ： 4 and emulsified, then dried at an inlet air temperature of 170 ℃ and a feeding rate of 10 ml / min for 10 s, then dried by spraying at an outlet temperature of 70 % for 10 s. The encapsulation efficiency of microcapsules prepared under the optimized condition was 77.4%, while the activity was maintained at more than 85%. The release rate 2 h after digestion in SGF was 8. 0%, while that 4 h after digestion in SIF was 81. 2%. The activity of microcapsules was more than 50% after being heated at 90 ℃ for 30 s, while was more than 90% after storage at 25 30 % for 10 months. However, after storage at 4 ℃ for 10 months, the activity decreased by only 5%. Conclusion The IgY microcapsules prepared by this process showed high biological activity and stability as well as sustained releasing properties with targeting effects on specific IgY.
出处
《中国生物制品学杂志》
CAS
CSCD
2016年第2期181-187,共7页
Chinese Journal of Biologicals
基金
广西科技攻关与新产品试制项目(桂科攻0719004-3B)
广西水产畜牧兽医局科技计划项目(桂渔牧科1304521)
广西南宁市科技成果转化与示范推广项目(20145190)资助
关键词
免疫球蛋白Y
微胶囊
缓释制剂
制备工艺
效果评价
IgY
Microcapsules
Sustained-released preparation
Preparation process
Effect evaluation
