摘要
目的:研究绵马贯众不同萃取部位的抑菌活性。方法:以70%乙醇冷浸72 h制备绵马贯众粗提物,液-液分离法制备石油醚、三氯甲烷、乙酸乙酯、正丁醇和水5个部位萃取物,用K-B纸片扩散法对大肠埃希菌、金黄色葡萄球菌、枯草杆菌、酵母菌、黄曲霉和黑根霉6种供试菌株的抑菌活性进行研究。结果:绵马贯众5个萃取部位均表现出一定的抑菌效果,且随着用药量的增加其抑菌活性总体上呈现出增强的趋势,其中以三氯甲烷萃取部位和乙酸乙酯萃取部位的抑菌活性较大,水萃取部位的抑菌活性最小。结论:不同部位对供试菌株的抑菌活性不同。
Objective： To study the antibacterial activities of different extract parts from Dryopteridis Crassirhizomatis Rhizoma. Method： The crude extracting of Dryopteridis Crassirhizomatis Rhizoma was extracted in 70% ethanol for 72 h,and the extracts of five fractions（ petroleum,chloroform,Eto Ac,n-Buo H and water）were prepared with liquid-liquid separation method. The antibacterial activities of different fractions on six strains（ Escherichia coli,Staphylococcus aureus,Bacillus subtilis,Saccharomyces cerevisiae,Aspergillus flavus and Rhizopus nigricans） were studied by K-B paper dispersion. Result： All five parts had certain antibacterial effects on test strains,and the overall antibacterial activities were increase with the increase of the drug dose. The chloroform and Eto Ac fractions had the stronger antibacterial activities,and the water fraction had the minimum antibacterial activities. Conclusion： Different fractions had different antibacterial activities on test strains.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第3期137-142,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
山西省自然科学基金项目(2011011036)
关键词
绵马贯众
萃取部位
细菌
真菌
抑菌活性
Dryopteridis Crassirhizomatis Rhizoma
extract fractions
bacteria
fungi
antibacterial activities
