摘要
目的研究高同型半胱氨酸血症对大鼠颈总动脉平滑肌细胞增殖及Caspase-12蛋白表达的影响。方法选取雄性SD大鼠40只,随机数字表法分为4组,每组10只。分别以不同剂量蛋氨酸饮食喂饲12周后分离颈总动脉。HE染色观察颈总动脉增殖情况;利用免疫荧光染色法观察并测定颈总动脉内增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)表达情况;利用免疫组织化学染色法及蛋白免疫印迹法检测Caspase-12蛋白表达情况。结果不同剂量蛋氨酸饮食导致大鼠颈总动脉不同程度增殖,颈总动脉内PCNA、Caspase-12蛋白表达增加,并呈浓度依赖性。结论高同型半胱氨酸血症通过激活血管平滑肌细胞内质网应激反应导致Caspase-12蛋白表达增加,加重血管损伤,促进平滑肌细胞增殖,进一步加重动脉粥样硬化。
Objective To test the effect of hyperhomocystinemia on the proliferation of rats carotid artery vascular smooth muscle cells and the expression of Caspase-12. Methods Forty male SD rats were divided into four groups randomly. The common carotid artery were separated after feeding with high methionine foods for three months and taken to HE staining for observing the proliferation of carotid arteries. The expression of proliferating cell nuclear antigen（ PCNA） was detected by immunofluorescence staining. The expression of Caspase-12 was tested by immunohistochemistry and Western blot. Results Different levels of methionine in the diet leaded to varying degrees of proliferation of common carotid artery smooth muscle cells and increased expression of PCNA and Caspase-12 in a dose-dependent manner. Conclusion Hyperhomocysteinemia can lead to increased expression of Caspase-12,which aggravates vascular injury through activation of endoplasmic reticulum stress response,promotes smooth muscle cell proliferation and further leads to atherosclerosis.
出处
《东南国防医药》
2016年第1期21-24,46,共5页
Military Medical Journal of Southeast China
