摘要
目的:建立大鼠急性酒精性肝损伤模型,探讨椇葛方对大鼠急性酒精性肝损伤的保护作用。方法:将60只SD大鼠随机分为6组,分别是空白组、模型组、易善复组(136.8 mg·kg-1·d-1)、椇葛方高剂量组(99.9mg·kg-1·d-1)、椇葛方中剂量组(33.3 mg·kg-1·d-1)、椇葛方低剂量组(11.1 mg·kg-1·d-1)。空白组灌胃蒸馏水,剂量为10 m L·kg-1,各给药组每日上午灌胃相应药物,每天给药1次,第4天开始除空白组外,其余各组每日上午正常给药,下午灌胃60%酒精10 m L·kg-1,连续酒精灌胃4 d。第4天酒精灌胃1 h后,颈总动脉取血,测定血清谷草转氨酶(AST)、谷丙转氨酶(ALT)以及甘油三酯(TG)含量。结果:与空白组相比,模型组大鼠血清ALT、TG含量显著升高(P<0.05),血清AST含量有升高趋势(P>0.05)。与模型组相比,椇葛方高剂量组血清ALT含量明显降低(P<0.01);椇葛方低剂量组血清TG含量明显降低(P<0.05),椇葛方高剂量组血清TG含量有降低趋势(P>0.05);椇葛方中剂量组血清AST含量明显降低(P<0.05),椇葛方高、低剂量组血清AST含量有降低趋势(P>0.05)。结论:椇葛方对急性酒精性肝损伤大鼠具有一定的肝脏保护作用。
Objective：To build an animal model of acute alcohol induced liver and study the protective effects of Juge Recipe on acute alcohol induced liver in rats. Method：The 60 rats were randomly divided into 6 groups：normal control group,model group,yishanfu group（136.8 mg·kg-1·d-1）,three dosage groups of Juge Recipe with low（11.1 mg·kg-1·d-1）,medium（33.3 mg·kg-1·d-1）and high（99.9 mg·kg-1·d-1）. The animals were given by distilled water or different dosage of Juge Recipe per day. On the fourth day,the animals were orally administrated（gavage）60% ethanol 10 m L·kg-1·d-1 for 4 consecutive days except the normal control group to build an animal model of acute alcohol induced liver. 1h after the last administration,the blood samples were collected for analysis of serum alanine aminotransferase（ALT）,aspartate aminotransferase（AST）and triglyceride（TG）. Results：Compared with normal control group,the ALT and TG levels of model group were remarkably increased（P0.05）,the level of AST was increased（P0.05）. Compared with model group,the high-dose of Juge Recipe group obviously reduced the serum level of ALT（P0.01）;The low-dose of Juge Recipe group remarkably reduced the serum level of TG（P0.05）,the high-dose of Juge Recipe group could reduce the serum level of TG（P0.05）;the medium-dose of Juge Recipe group reduced the serum level of AST obviously（P0.05）,the other dose of Juge Recipe could reduce the serum level of AST（P0.05）. Conclusion：Juge Recipe has some protective effects on acute alcohol induced liver in rats.
出处
《辽宁中医药大学学报》
CAS
2016年第2期27-29,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
北京市"十病十药研发"项目(Z141100002214008)
