摘要
【目的】探讨微生物源和化学农药对水稻稻瘟病相关抗性酶系活性的影响,为研发安全、绿色的生物农药提供理论依据。【方法】于水稻3叶1心期进行微生物源农药[腐片镰刀菌（Fusarium solani）次生代谢产物,WS]和化学农药苯并噻二唑（BTH）诱抗处理,以喷洒蒸馏水为对照（空白对照CK1和阳性对照CK2）,采用外接稻瘟病病菌法测定相关抗性酶系活性及植株抗病性。【结果】接种稻瘟病菌后0-9 d,WS处理的苯丙氨酸解氨酶（PAL）、过氧化物酶（POD）和多酚氧化酶（PPO）活性及BTH处理的PAL和POD活性均高于CK2,BTH处理对提高PAL活性的效果优于WS处理,但WS对提高PPO活性的持续性较优;两种诱抗剂前期（0-3 d）均可提高叶片过氧化氢酶（CAT）活性,但后期CAT活性均低于CK2;接种稻瘟病菌后各处理的肉桂醇脱氢酶（CAD）活性均无明显变化。WS和BTH诱导处理后,水稻病株率、病斑数及病情指数均降低,其中WS处理的发病情况极显著低于CK（P〈0.01）,诱抗效果达56.21%,比BTH处理高14.78%（绝对值）。【结论】WS和BTH对诱导水稻抗稻瘟病均有一定效果,且WS的诱抗效果整体上优于BTH。
【Objective】The present experiment was conducted to investigate effect of biological and chemical pesticides on rice blast resistance-related enzyme activity, in order to provide theoretical basis for development of safe and green bio-pesticides. 【Method】The microbial pesticide（Fusarium solani secondary metabolite, WS）, chemical pesticide（benzothiadiazole, BTH） and distilled water（blank control, CK1 and positive control, CK2） was sprayed on rice at the growth stage of three leaves and one heart. Then the resistance-related enzyme activity and resistance to rice blast were determi-nated after being externally inoculated with rice blast pathogen. 【Result】In 0-9 days after being externally inoculated with rice blast pathogen, the activities of phenylalanine ammonia-lyase（PAL）, peroxidase（POD）, and polyphenol oxidase（PPO） in the rice treated by WS, and activities of PAL and POD in the rice treated by BTH were higher than those in CK2. BTH treatment had better improving effects on PAL activity than WS treatment, but WS treatment had better continuous improving effects on PPO activity than BTH treatment. In the early stage of WS and BTH treatments（0-3 days）,the catalase（CAT） activity could be improved, but in the late stage, the CAT activity in the leaves was lower than that of CK2. Moreover, each treatment couldn＇t change obviously cinnamyl-alcohol dehydrogenase（CAD） activity after inoculation. And the rate of diseased plant, number of lesions, disease index were all decreased after WS and BTH treatments.The disease index of rice treated by WS was significantly lower than that of CK（P〈0.01）, and the induced-resistance effect of WS was up to 56.21%, which was 14.78% higher than that of BTH（absolute value）. 【Conclusion】WS and BTH have a certain induced effects on rice blast-resistance, but induced effect of WS is superior to that of BTH.
出处
《南方农业学报》
CAS
CSCD
北大核心
2016年第1期24-28,共5页
Journal of Southern Agriculture
基金
辽宁省"百千万人才工程"入选项目(2012921065)
辽宁省自然科学基金项目(2015020801)
