摘要
目的采用HPLC法同时测定复方天麻钩藤口腔崩解片(天麻、钩藤、白芍、当归)中天麻素、芍药苷和阿魏酸的含有量。方法该药物50%甲醇提取物的分析采用C18反相色谱柱;流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长为230 nm(天麻素、芍药苷)和320 nm(阿魏酸)。结果天麻素、芍药苷、阿魏酸分别在0.153~1.377μg、0.298 6~2.687 4μg、0.022 68~0.204 12μg范围内呈良好的线性关系,平均回收率分别为98.4%、97.9%、97.4%。结论该方法可用于复方天麻钩藤口腔崩解片的质量控制。
AIM To develop an HPLC method for simultaneously determining the contents of gastrodin,paeoniflorin and ferulic acid in Fufang Tianma Gouteng Orally Disintegrating Tablets( Gastrodiae Elatae Rhizoma,Uncariae Ramulus cum Uncis,Paeoniae Lactilorae Radix and Angelicae Sinensis Radix). METHODS The analysis of 50% methanolic extract of this drug was performed on C18 ODS column,mobile phase was acetonitrile-0. 1%phosphoric acid with gradient elution,and detection wavelengths were set at 230 nm for gastrodin and paeoniflorin,and 320 nm for ferulic acid. RESULTS Gastrodin,paeoniflorin and ferulic acid displayed good linear relationships within the ranges of 0. 153-1. 377 μg,0. 298 6-2. 687 4 μg and 0. 022 68-0. 204 12 μg,and their average recoveries were 98. 4%,97. 9% and 97. 4%,respectively. CONCLUSION This method can be applied to the quality control of Fufang Tianma Gouteng Orally Disintegrating Tablets.
出处
《中成药》
CAS
CSCD
北大核心
2016年第2期315-318,共4页
Chinese Traditional Patent Medicine
基金
国家自然科学基金资助项目(81173165)
太原市科学技术局资助项目(11014918)
关键词
复方天麻钩藤口腔崩解片
天麻素
芍药苷
阿魏酸
HPLC
Fufang Tianma Gouteng Orally Disintegrating Tablets
gastrodin
paeoniflorin
ferulic acid
high performance liquid chromatogram(HPLC)