摘要
目的：建立同时测定香连化滞丸中芍药苷、橙皮苷、黄芩苷、巴马汀、小檗碱、甘草酸和厚朴酚7种成分含量的方法。方法：采用高效液相色谱法。色谱柱为Agilent Porlshell 120 SB C18,流动相为90%乙腈（A）-5%乙腈（含0.1%磷酸,B）（梯度洗脱）,流速为1.0 ml/min,柱温为30℃,进样量为3μl,检测波长分别为芍药苷230 nm（0~10.0 min）、橙皮苷283 nm（10.1~13.0 min）、黄芩苷277 nm（13.1~14.4 min）、巴马汀（以盐酸巴马汀计）和小檗碱（以盐酸小檗碱计）265 nm（14.5~20.0 min）、甘草酸（以甘草酸铵计）250 nm（20.1~25.0 min）、厚朴酚290 nm（25.1~60.0 min）。结果：芍药苷、橙皮苷、黄芩苷、巴马汀（以盐酸巴马汀计）、小檗碱（以盐酸小檗碱计）、甘草酸（以甘草酸铵计）和厚朴酚的检测进样量线性范围分别为0.013 88~0.694 5、0.039 93~1.996 8、0.070 08~0.389 3、0.006 48~0.324 0、0.010 52~0.526 2、0.008 816~0.440 7、0.007 224~0.361 2μg（r≥0.999 5）;精密度、稳定性、重复性试验的RSD〈2.0%;平均加样回收率分别为97.47%~102.88%、102.06%~102.81%、97.91%~100.80%、97.53%~101.60%、97.54%~100.68%、96.23%~99.00%、97.91%~101.44%,RSD分别为1.91%、0.25%、1.25%、1.66%、1.15%、1.11%、1.36%（n=6）。结论：该方法简单、快速、准确,可用于香连化滞丸的质量控制。
OBJECTIVE：To establish a method for the contents determination of paeoniflorin,hesperidin,baicalin,palmatine,berberine,glycyrrhizic acid,magnolol in Xianglian huazhi pill. METHODS：HPLC was conducted on the column of Agilent Porlshell 120 SB-C18 with mobile phase of 90% acetonitrile（A）-0.1% phosphoric acid 5% aqueous acetonitrile（B）（gradient elution）at a flow rate 1.0 ml/min,the column temperature was 30 ℃,the injection volume was 3 μl,and the detection wavelength was 230 nm for paeoniflorin（0-10.0 min）,283 nm for hesperidin（10.1-13.0 min）,277 nm for baicalin（13.1-14.4 min）,265 nm for palmatine and berberine（14.5-20 min）,250 nm for glycyrrhizic acid（20.1-25.0 min） and 290 nm for magnolol（25.1-60.0min）. RESULTS：The linear range was 0.013 88-0.694 5 μg for paeoniflorin,0.039 93-1.996 8 μg for hesperidin,0.070 08-0.389 3μg for baicalin,0.006 48-0.324 0 μg for palmatine（counted by palmatine hydrochloride）,0.010 52-0.526 2 μg for berberine（counted by berberine hydrochloride）,0.008 816-0.440 7 μg for glycyrrhizic acid（counted by ammonium glycyrrhizinate）and 0.007 224-0.361 2 μg for magnolol（r≥0.999 0）;RSDs of precision,stability and reproducibility tests were lower than 2.0%;average recoveries were97.47%-102.88%（RSD=1.91%,n=6）,102.06%-102.81%（RSD=0.25%,n=6）,97.91%-100.80%（RSD=1.25%,n=6）,97.53%-101.60%（RSD=1.66%,n=6）,97.54%-100.68%（RSD=1.15%,n=6）,96.23%-99.00%（RSD=1.11%,n=6） and97.91%-101.44%（RSD=1.36%,n=6）,respectively. CONCLUSIONS：The method is simple,rapid and accurate,and can be used for the quality control of Xianglian huazhi pill.
出处
《中国药房》
CAS
北大核心
2016年第6期840-843,共4页
China Pharmacy
关键词
香连化滞丸
芍药苷
橙皮苷
黄芩苷
巴马汀
小檗碱
甘草酸
厚朴酚
含量测定
Xianglian huazhi pill
Paeoniflorin
Hesperidin
Baicalin
Palmatine
Berberine
Glycyrrhizic acid
Magnolol
Content determination
