摘要
目的通过观察针灸治疗前后环磷酰胺(CTX)化疗模型小鼠血清中白介素-7(IL-7)和白介素-18(IL-18)含量的变化,探讨针灸改善化疗后所导致免疫抑制的分子生物学机制。方法选用清洁级昆明种雄性小鼠80只,按体重随机分为空白组、模型组、针刺组、艾灸组,每组20只。除空白组外,其余各组均腹腔注射CTX制备小鼠骨髓抑制模型。针刺组和艾灸组选取大椎、膈俞、肾俞、足三里进行针刺和艾灸治疗,空白组、模型组每日陪同抓取和固定,不做任何治疗。依次经过治疗后3d和5 d,在每组中分两次分别处死10只小鼠,摘眼球取血,采用ELISA法检测血清中IL-7和IL-18的含量。结果治疗3 d至5 d,针刺组和艾灸组白细胞均开始回升,且针刺组疗效优于艾灸组;在3 d组和5 d组中,针刺组和艾灸组血清中IL-7含量均高于模型组(P<0.05),且针刺组疗效优于艾灸组(P<0.05);在3 d组和5 d组中,针刺组和艾灸组血清中IL-18含量均高于模型组(P<0.05),且在5 d组中,针刺组疗效优于艾灸组(P<0.05)。结论针刺和艾灸可以明显上调CTX化疗小鼠血清中IL-7、IL-18的含量,减轻CTX引起的免疫抑制,提高机体的免疫功能。
Objective To discuss the molecular biological mechanism of acupuncture and moxibustion in improving immunosuppression due to chemotherapy, by observing the effect of acupuncture and moxibustion on the serum contents of interleukin（IL）-7 and IL-18 in mice undergone chemotherapy with Cyclophosphamide（CTX）. Method Eighty SPF male Kunming rice were randomized into a blank control group, a model group, an acupuncture group, and a moxibustion group according to the weight, 20 mice in each group. All of the groups except the blank control received intraperitoneal injection with CTX to prepare the mouse model of myelosuppression. The acupuncture group and moxibustion group respectively received acupuncture and moxibustion intervention at Dazhui（GV14）, Geshu（BL17）, Shenshu（BL23） and Zusanli（ST36）, while the blank control group and model group were only treated with grasping and fixing. Respectively after 3-day and 5-day interventions, 10 mice from each group were sacrificed each time to collect blood via removing eyeballs, and the enzyme linked immunosorbent assay（ELISA） was used to examine the serum contents of IL-7 and IL-18. Result After 3-day and 5-day interventions, the white blood cells increased in both acupuncture and moxibustion groups, and the efficacy of the acupuncture group was more significant than that of the moxibustion group; despite 3-day intervention or 5-day intervention, the serum contents of IL-7 in both acupuncture and moxibustion groups were significantly higher than that in the model group（P〈0.05）, and the efficacy of the acupuncture group was more significant than that of the moxibustion group（P〈0.05）; after 3-day and 5-day interventions, the serum contents of IL-18 in both acupuncture and moxibustion groups were significantly higher than that in the model group（P〈0.05）, and the efficacy of the acupuncture group was more significant than that of the moxibustion group after 5-day intervention（P〈0.05）. Conclusion Acupuncture and moxibustion can significantly up-regulate the serum contents of IL-7 and IL-18 in mice undergone CTX chemotherapy, mitigate immunosuppression caused by CTX, and improve immune function of the body.
出处
《上海针灸杂志》
2016年第2期223-226,共4页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金项目(81072884)
