摘要
为控制贵州省鸭疫里默氏杆菌(RA)的流行,本研究以实验室分离保存的血清2型RA地方优势流行株为菌种,制备了稳定性和安全性较好的甲醛油乳剂灭活疫苗,以其免疫麻鸭后对其抗体滴度进行检测,并于免疫后以RA分离株进行免疫保护攻毒试验。结果显示,该灭活疫苗诱导麻鸭产生的抗体滴度可达1∶3 200,免疫保护率达87.5%,高于商品化的同类灭活疫苗(62.5%)。结果表明利用贵州地区流行的优势血清型RA菌株所制备的疫苗,对防治RA病效果明显。
To efficiently control the prevalence of duck Riemerella anatipestifer（RA） disease in Guizhou province,the dominant serotype 2 of R.anatipestifer isolate was used to prepare formaldehyde oil emulsion inactivated vaccine in this study.The produced vaccine was confirmed to be stable and safe,and able to induce the antibody titer up to 1 ∶3,200.The immune protection rate sheldrakes was 87.5%,which was superb to the 62.5% protection for the ducks by commercial R.anatipestifer inactivated vaccine.These results demonstrated that inactivated vaccine produced by the dominant R.anatipestifer isolate provided efficient immunity and protection for ducks.
出处
《中国预防兽医学报》
CAS
CSCD
北大核心
2016年第2期151-154,共4页
Chinese Journal of Preventive Veterinary Medicine
基金
贵州省科技计划项目"水禽免疫增强剂创制与开发关键技术研究"(黔科合计省合[2013]7004号)
教育部促美大项目"表达鸭疫里氏杆菌外膜蛋白Omp A重组新城疫病毒的构建及免疫保护评价"(教外司美[2014]2029)
贵州省重大专项课题"三穗鸭主要疫病防控与健康养殖关键技术集成应用"(黔科合重大专项字[2012]6004)
关键词
鸭疫里默氏杆菌
血清2型
灭活疫苗
免疫保护
duck Riemerella anatipestifer
serotype 2
inactivated vaccine
immune protection
