摘要
目的脂代谢紊乱可导致雄性不育,但其影响精子发生及睾丸微环境的机制尚不明确。文中旨在探讨高脂饮食对雄性小鼠生殖功能以及睾丸内氧化低密度脂蛋白(Ox-LDL)表达变化的影响。方法 16只8周龄雄性C57BL/6J小鼠随机数字表法分为正常饮食组和高脂饮食组,每组8只。分别给予标准饲料与高脂饲料维持饮食干预连续喂养16周。检测血清三酰甘油(TG)、总胆固醇(TC)、氧化低密度脂蛋白(Ox-LDL)以及睾酮水平;取附睾尾测定精子数量和活力,HE染色观察睾丸结构变化,免疫组织化学分析小鼠睾丸组织中Ox-LDL的免疫定位和表达变化。结果与正常饮食组相比,高脂饮食组小鼠体重显著增加(P<0.05),血清TG差异无统计学意义(P>0.05),高脂饮食组较正常饮食组血清TC升高[(2.31±0.33)mmol/L vs(6.54±0.31)mmol/L,P<0.01],血清Ox-LDL升高[(0.32±0.03)mg/L vs(0.44±0.06)mg/L,P<0.01],血清睾酮降低[(3.64±0.43)mg/L vs(0.40±0.14)mg/L,P<0.01],小鼠精子浓度降低[(9.95±0.75)106/m L vs(5.66±1.51)106/m L,P<0.01]和精子活动率降低[(54.69±17.84)%vs(32.48±5.80)%,P<0.01]。HE染色显示高脂饮食组较正常饮食组睾丸间质细胞、睾丸生精细胞与腔内精子数量明显减少,同时免疫组织化学显示Ox-LDL定位于睾丸间质细胞周围且表达明显升高(P<0.01)。结论睾丸局部微环境中,Ox-LDL升高可能抑制了Leydig细胞的睾酮合成,进而影响了睾丸生精功能。
Objective Lipid metabolism disorder can lead to male sterility,but its mechanism of affecting spermatogenesis and testis microenvironment remains unclear. This study aimed to investigate the effects of high fat diet on reproductive function and the expression of Ox-LDL within testis in male mice. Methods 16 C57 BL /6 mice aged 8 weeks were divided into two groups by random number table method. High fat diet group was fed with high fat diet while normal fat diet group was fed with normal fat diet. At the end of 16 weeks,the levels of TG,TC,Ox-LDL and testosterone in serum were measured. The sperm concentration and motility from caudal epididymis were analyzed. The testis structure was observed by HE staining. The localization and expression of Ox-LDL in testis were detected by immunohistochemical technique. Results Compared with the normal diet group mice,the body weight（ P〈0. 05） and the serum levels of TC [（ 2. 31 ± 0. 33） mmol / L vs（ 6. 54 ± 0. 31） mmol / L,P〈0. 01],Ox-LDL [（ 0. 32 ± 0. 03） mg / L vs（ 0. 44 ± 0. 06） mg / L,P〈0. 01] increased in high fat diet group mice significantly. The serum level of TG was of no significant difference（ P〈0. 05）. The serum level of testosterone[（ 3. 64 ± 0. 43） mg / L vs（ 0. 40 ± 0. 14） mg / L,P〈0. 01],the sperm concentration[（ 9. 95 ± 0. 75） 106/mL vs（ 5. 66 ± 1. 51） 106/ mL,P〈0. 01]and the sperm motility[（ 54. 69 ± 17. 84） % vs（ 32. 48 ± 5. 80） %,P〈0. 01]decreased in high fat diet group significantly. HE staining also showed that the amount of Leydig cells,spermatids and spermatozoons reduced obviously in high fat diet group. Immunohistochemistry staining showed that Ox-LDL was mainly distributed around Leydig cells of mice testis. The expression of Ox-LDL in high fat diet group increased significantly（ P〈0. 01）. Conclusion The expression of Ox-LDL in Leydig cells of high fat diet C57 BL /6J mice increases significantly,which may inhibit testosterone biosynthesis and affect spermatogenic function.
出处
《医学研究生学报》
CAS
北大核心
2016年第2期133-137,共5页
Journal of Medical Postgraduates
基金
国家重大基础研究发展计划(2013CB945200)
