摘要
目的研究高压氧(HBO)后处理对大鼠皮瓣缺血再灌注损伤炎性反应的影响。方法 36只雄性SD大鼠按数字表法随机分为假手术组(SH组)、缺血再灌注组(IR组)、高压氧后处理组(HBO+IR组),每组12只。麻醉后在大鼠腹部形成以右侧腹壁下浅动脉为蒂的扩大腹部皮瓣,IR组及HBO+IR组皮瓣用显微血管夹阻断血供3 h后恢复血供。术后12 h开始给予相应压力的HBO后处理,2次/d,连续7次,每次1 h,2次之间间隔10 h。其他2组处于常压空气中。术后第8天处死大鼠,沿皮瓣血管轴在皮瓣中段取材。HE染色观察组织炎症浸润,RT-PCR法测定组织ICAM-1、MMP-9的表达。结果 HE染色显示:相同取材部位HBO+IR组皮瓣组织炎性细胞浸润较IR组轻。与IR组相比,HBO+IR组ICAM-1、MMP-9明显降低,差异有统计学意义(P<0.05)。结论 HBO后处理可通过抑制ICAM-1、MMP-9表达,减轻移植术后缺血再灌注损伤造成的炎性反应,提高皮瓣成活率。
Objective To investigate the effects of hyperbaric oxygen （HBO） post processing on inflammatory reaction induced by ischemia-reperfusion （IR） injury after skin flap transplantation in rats. Methods Thirty-six male Sprague-Dawley （SD） rats were randomly divided into 3 groups ： sham-surgery （ SH ） group, ischemia-reperfusion （IR） group and the HBO post processing ＋ ischemia-repeffusi0n group, with 12 rats in each group. The animal models with abdominal pedicle skin flap were established, then the rats in HBO ＋ IR group received HBO post processing. On the 8th day after surgery, the rats were sacrificed and the tissue specimens were collected at the proximal, intermediate and distal portions of the flaps. The tissue inflammatory infiltration was detected by HE staining and the expression levels of intercellular adhesion molecule-1 （ICAM-1） and metalloproteinases-9 （MMP-9） were detected by quantitative Real-time PCR （RT-PCR）. Results HE staining showed that inflammatory cell infiltration in HBO ＋ IR group was slighter than that at the same dissected position in IR group, moreover, the expression levels of ICAM-1 and MMP-9 in HBO ＋ IR group were significantly lower than those in IR group （ P 〈 0. 05）. Conclusion Hyperbaric oxygen post processing may alleviate inflammatory reaction induced by IR injury after skin flap transplantation in rats through inhibiting the expression levels of MMP-9 and ICAM-1 so as to increase the survival rate of skin flap.
出处
《河北医药》
CAS
2016年第1期24-26,共3页
Hebei Medical Journal
关键词
高压氧
细胞间黏附分子
基质金属蛋白酶9
皮瓣
hyperbaric oxygen
intercellular adhesion molecule
metalloproteinases-9
skin flap
