摘要
目的探讨非小细胞肺癌患者外周血中人类肺特异性X蛋白(Lunx)mRNA的表达情况。方法选取2012年6月至2014年10月间收治的60例非小细胞肺癌患者作为研究组,选取同期健康体检人员(健康对照组)40名及肺部良性疾病者(肺部良性疾病组)30例作为对照。采用实时荧光定量反转录聚合酶链反应(FQ-RT-PCR)法定量检测患者外周血中Lunx mRNA的表达情况,并分析Lunx mRNA表达情况及其与临床病理特征的关系。结果研究组患者Lunx mRNA表达阳性者34例(56.7%)。健康对照组及肺部良性疾病组患者Lunx mRNA表达阴性。Lunx mRNA表达与研究组患者临床分期相关(P<0.05),但与患者性别、病理分型、性别及分化程度等均无关(均P>0.05)。结论应用FQ-RT-PCR法定量检测非小细胞肺癌患者外周血中Lunx mRNA敏感性及特异性均较高,从而为临床早期诊断肺癌微转移提供一定帮助,并指导临床病理分期及治疗方案的选择,改善其预后。
Objective To explore the value of lung specific X protein （Lunx） mRNA in peripheral blood of non-small cell lung cancer. Methods From June 2012 to October 2014 in Longgang Central Hospi- tal, 60 non-small cell lung cancer patients were enrolled in the study as the study group, 40 cases of healthy people healthy and 30 patients with benign lung disease as the control group. FQ-RT-PCR assay was used to detect the Lunx mRNA expression. The relationship between Lunx mRNA expression and the clinical factors was analyzed. Results 34 cases （56. 7% ） of Lunx mRNA showed positive in the study group. The control group showed negative expression of Lunx mRNA. Lunx mRNA expression in the study group was related to tumor stages（P 〈0. 05）, but was not to gender, pathological type, sex and degree of differentiation（ P 〉 0. 05）. Conclusions FQ-RT-PCR assay for quantification Lunx mRNA in peripheral blood of non-small cell lung cancer patients has sensitivity and specificity, and thus provide help for the clinical diagnosis of early stage lung cancer micrometastases.
出处
《中国肿瘤临床与康复》
2016年第2期156-158,共3页
Chinese Journal of Clinical Oncology and Rehabilitation
