摘要
目的：探讨D-松醇原药对大鼠肝脏的毒性。方法：4-6周龄健康清洁级SD大鼠80只（雌雄各半）,随机分成D-松醇原药低剂量组、中剂量组、高剂量组及对照组,每组20只,雌雄各10只,低、中、高剂量组分别喂饲390.63 mg/kg、1 562.50 mg/kg和6 250.00 mg/kg的D-松醇原药饲料,对照组喂饲普通标准饲料,连续喂饲90 d;观察4组大鼠的一般情况、体重变化;第90天时,各组大鼠心脏取血检测天冬氨酸氨基转换酶（AST）、丙氨酸氨基转换酶（ALT）、碱性磷酸酶（ALP）、总蛋白（TP）、白蛋白（ALB）、球蛋白（GLO）、血糖（GLU）、总胆固醇（CHO）、总胆红素（TBLL）、胆碱酯酶（CHE）10项肝功能指标,取各组大鼠肝脏计算肝脏脏体比及观察肝脏病理组织学改变。结果：D-松醇原药低、中、高剂量组雄性大鼠实际染毒量分别为33.58 mg/kg、149.39 mg/kg、543.98 mg/kg;雌性大鼠实际染毒剂量分别为30.88 mg/kg、127.06 mg/kg、483.88 mg/kg。高剂量组雌性大鼠GLO、TP低于对照组（P〈0.05）;中、高剂量组雌性大鼠CHE高于对照组（P〈0.05）;中、高剂量组雄性大鼠TBLL高于对照组（P〈0.05）。结论：D-松醇原药90 d经口喂养最大无作用剂量,雄性为30.88 mg/kg、雌性为33.58 mg/kg。雄性大鼠摄入D-松醇原药127.06 mg/kg以及雌性大鼠摄入D-松醇原药149.39 mg/kg时可以引起大鼠肝脏损伤。
Objective： To investigate D-pinitol technical grade toxicity on rats liver. Methods： Eighty healthy SD rats of 4 to 6 weeks of age （equal male and female number）, randomly divided into D-pinitol technical grade low, medium and high dose groups and control group, with 10 male and 10 female rats each; low, medium and high dose groups were fed with 390.63 mg/kg, 1 562.50 mg/kg and 6 250.00 mg/kg D-pinitol technical grade, control group was fed with normal standard stuff, continuous fed for 90 days ; observing general condition of 4 groups and weight change ; on 90th day, heart blood testing on liver function index of AST, ALT, ALP, TP, ALB, GLO, GLU, CHO, TBLL and CHE. Extracting rats＇liver to calculate liver ratio and liver pathological changes. Results： The actual intoxicated amount of male rats in low, middle and high dose group were 33.58 mg/kg, 149.39 mg/kg and 543.98 mg/kg; and the actual intoxicated amount of female rats were 30.88 mg/kg, 127.06 mg/ kg, 483.88 mg/kg. Globulin （GLO）, total protein （TP） of female rats in high dose group was less than that of control group （ P 〈 0.05 ）. Cholinesterase （CHE） of female rats in middle and high dose group was higher than that of control group （ P 〈 0.05 ）. Conclusion： D-pinitol technical grade fed for 90 days no-effect levels were as follows ： 127.06 mg/kg for male rats and 149.39 mg/kg for femal rats of D-pinitol technical grade can would cause liver damage in rats.
出处
《贵阳医学院学报》
CAS
2016年第2期190-193,共4页
Journal of Guiyang Medical College
