摘要
目的检测肥胖大鼠网膜脂肪组织KLF4及KLF7 m RNA表达水平,分析其与炎症的相关性。方法 Wistar健康雄性大鼠高脂喂养,诱导肥胖模型。高脂喂养后第4、10周检测大鼠血脂、血糖水平;ELISA法测定血浆中TNF-α、LPT、APN水平;10周后,q RT-PCR法检测网膜脂肪组织KLF4、KLF7及NF-κB炎症信号通路关键因子m RNA表达水平。结果高脂喂养4周后,实验组大鼠体质量开始显著高于对照组,第10周实验组大鼠体质量进入平台期,但仍高于对照组(P<0.05)。高脂喂养第4及第10周,实验组大鼠血浆FFA、Glu、TG、TC、LDL、TNF-α水平高于对照组,LPT、APN水平低于对照组(P<0.05)。网膜脂肪组织中,实验组TLR9、KLF4 m RNA表达水平低于对照组,KLF7、SRC和IL-6 m RNA表达水平高于对照组;TLR9与血浆FFA负相关,与KLF4正相关;KLF4与SRC、NF-κB负相关;KLF7与TLR4、SRC、NF-κB和IL-6正相关,与KLF4负相关(P<0.05)。结论肥胖状态下,高水平的FFA一方面可与TLR4结合上调KLF7表达,促进炎症因子表达,导致释放组织发生炎症;同时,可抑制TLR9水平而下调KLF4表达,减弱KLF4对炎症信号通路关键因子的抑制作用,从而促进炎症反应。
The purpose of this study is to detect KLF4 and KLF7 mRNA expression of the omental adipose tissue, and analyze the relationship between KLF4, KLF7 and inflammatory in obese rats. Healthy male Wistar rats were fed with high fat diet to induce obesity rat model. The levels of FFA, Glu, TG, TC, LDL and HDL were detected in the 4th and the 10th week after the high fat diet; the levels of TNF-α, LPT, and APN in plasma were tested by ELISA. Ten weeks after, qRT-PCR was applied to detect the expression of KLF4, TLR7 and the mRNA expression levels of NF-κB inflammatory signaling pathway key factors. After 4 weeks＇ high fat diet, the weight of the rats in the experimental group was significantly higher than that of the control group （P〈0.05）, and 10 weeks after, the experimental group rat weight went to the plateau phase, which still higher than the control group in the 10th week （P〈 0.05）. In the 4th and 10th weeks, the plasma FFA, Glu, TG, TC, LDL and TNF-α of experimental group rats were higher than those of the control group, while LPT and APN levels were lower than those of the control group （P〈 0.05）. In the the omental adipose tissue, the mRNA expression levels of TLR9, KLF4 and NF-κB in the experimental group were lower than those in the control group, while KLF7, SRC and IL-6 mRNA expression levelwere higher than those of the control group. TLR9 was negatively related to FFA levels in plasma, while positively correlated with KLF4; KLF4 was negatively related to SRC and NF-κB; KLF7 was negatively correlated with TLR4, SRC, NF-κB and IL-6, while negatively correlated to the KLF4 （P〈0.05）. In the state of obese, high levels of FFA can combine with TLR4 to up-regulate KLF7 expression, promoting theexpression of critical inflammatory factor; meanwhile, high levels of FFA can aggravate adipose tissue inflammation by inhibiting TLR9 level to down-regulate KLF4 expression, abating KLF4 inhibition of critical factor of inflammatory signaling pathway to accelerate inflammatory response.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2016年第3期204-209,共6页
Immunological Journal
基金
国家自然科学基金(81360142)
石河子大学重大科技攻关计划项目(gxjs2012-zdgg02)
石河子大学高层次人才科研启动资金专项(RCZX201230)
石河子大学大学生研究训练计划(SRP2015246)
兵团应用基础研究计划(2015AG016)
