摘要
目的筛选抗癫痫药物及研究该药物在癫痫方面的相关作用。方法将实验小鼠随机分为模型组、阳性对照组、治疗药物组。其中,模型组小鼠一次腹腔注射给予戊四唑(90mg·kg-1),造成戊四唑急性点燃癫痫小鼠模型。实验小鼠在分别给予药物(银杏叶水提物、石菖蒲、曲克芦丁、金纳多、野黄芩苷、阿魏酸、七叶皂苷钠)及丙戊酸钠后30min,给予PTZ,通过观察小鼠的潜伏期来初步筛选药物的抗癫痫作用。结果与模型组相比,银杏水提物与石菖蒲未能延长癫痫小鼠的潜伏期,曲克芦丁、阿魏酸、金纳多、野黄芩苷虽能延长癫痫发作的潜伏期,但无显著性差异(P<0.05)。而七叶皂苷钠高剂量组、丙戊酸钠组均延长了癫痫发作的潜伏期,其中七叶皂苷钠(2.6mg·kg-1)与模型组相比有显著性差异(P<0.01),与阳性对照组相比,无显著性差异(P>0.05),说明七叶皂苷钠具有一定的抗癫痫作用。结论七叶皂苷钠具有一定的抗癫痫作用,且对癫痫后的肌损伤及运动协调能力具有一定的恢复作用。
OBJECTIVE To screening of antiepileptic drugs and study the drug related role in epilep- sy. METHODS The experimental mice were randomly divided into model group, positive control group, the treat- ment group. Among these, the model group mice received intraperitoneal injection of PTZ （90mg · kg-1） to produce acute PTZ lighting of epilepsy mouse model. In experimental group mice were given drugs （ ginkgo biloba extract wa- ter, stone calamus, QuKe rutin, skinner, wild baicalin, ferulic acid sodium, sodium aescinate and 30 minutes after val- proate, gave the PTZ, observing the incubation period of the mice to a preliminary screening of antiepileptic drugs. RESULTS The experimental results showed that, compared with model group, ginkgo biloba extract and wa- ter stone calamus failed to extend the incubation period of epilepsy in mice. QuKe rutin, ferulic acid, skinner, wild ba- icalin can extend the incubation period of epilepsy in mice, but there was no significant difference （P 〉 0. 05 ）. Each dose group,and seven Ye Zao glycosides sodium valproate group were prolonged the incubation period of epileptic seizures,seven Ye Zao glycosides sodium （2. 6mg · kg-1 ） compared with model group have significant difference, （P 〈0. 01 ）, compared with the positive control group, there was no significant difference （P 〉 0. 05 ）, showed that sodium aescinate possessed antiepileptic effect. CONCLUSION Sodium aescinate have a certain antiepileptic effect, and play a certain role in the recovery of muscle injury and motor coordination ability.
出处
《海峡药学》
2016年第1期20-22,共3页
Strait Pharmaceutical Journal
基金
福建省中医药科研项目(wzzy201308)
关键词
抗癫痫
戊四唑
丙戊酸钠
七叶皂苷钠
Anticonvulsant
Pentylenetetrazole
Valproate
Sodium aescinate
