摘要
目的建立高效液相色谱-质谱联用法测定人血浆中奥美拉唑的浓度。方法以泮托拉唑为内标,血浆样品经乙腈沉淀、取上清稀释后,进样,经HPLC-MS/MS分析。采用Ultimate XB-C18（2.1 mm×150 mm,3μm）,流动相甲醇（0.1%甲酸）∶水（0.1%甲酸）=（70∶30,V/V）;流速：0.2m L·min-1,柱温：40℃,电喷雾离子源（ESI）,以多离子反应监测方式（MRM）进行正离子监测,奥美拉唑和内标泮托拉唑的定量分析离子对分别为m/z 346.2→m/z 198.0和m/z 384.0→m/z 200.0。结果奥美拉唑的线性范围为5.00-2000.00μg·L-1,定量下限为5.00μg·L-1。方法回收率在96.4%-107.9%,日内和日间精密度（RSD）均小于15%。结论该方法操作简便、灵敏、准确、快速,适用于奥美拉唑的人体药代动力学研究。
OBJECTIVE To establish a high performance liquid chromatography tandem mass spectrometry （HPLC-MS/MS） method for the determination of Omeprazole in human plasma. METHODS Human plasma sam- ples were precipitated with acetonitrile and determined by HPLC-MS/MS using pantoprazole as the internal stand- ard. The separation was carried out on a Ultimate XB-C18 （2. 1 mm x 150mm ,3 μm） with a mobile phase of methanol- water （ including 0. 1% formic acid） （70：30） and the flow rate was 0. 2mL · min -1. ESI was performed in the MRM positive mode with using target ions at m/z 346.2-m/z 198.0 （Omeprazole） and m/z 384. 0--m/z 200. 0 （panto- prazole）. RESULTS The calibration curve of Omeprazole was obtained in the range of 5.00 - 2000. 00μg· L-1 with good linearity （r 〉0. 99）. The LLOQ of Omeprazole was 5μg · L-1. The method recovery was between 96. 4% and 107.9% ,and the intra and inter day RSD were less than 15%. CONCLUSION This method is convenient, sensitive, accurate and fast, which is suitable for human pharmacokinetic studies of Omeprazole.
出处
《海峡药学》
2016年第1期204-207,共4页
Strait Pharmaceutical Journal
关键词
奥美拉唑
高效液相色谱串联质谱法
血药浓度
Omeprazole
Liquid chromatography tandem mass spectrometry
Plasma concentration of drug
