摘要
为探索与烟叶中茄尼醇含量相关基因的遗传模式,以低茄尼醇含量烟草种质Maryland609为母本、高茄尼醇含量烟草种质K326为父本配制杂交组合,建立P_1、P_2、F_1、F_2的4个世代遗传分析群体,利用UPLC（超高效液相色谱）进行茄尼醇的定量检测,通过＂主基因＋多基因＂混合遗传模型多世代联合分析对与茄尼醇含量相关的基因进行遗传分析。结果表明,Maryland609×K326组合的茄尼醇含量受2对等显性主基因＋加性显性多基因（E6）控制,2对主基因的加性效应和显性效应值相等,均为负值,多基因的显性效应大于加性效应;主基因的遗传率分别为33.61%和53.15%,多基因遗传率分别为30.01%和13.64%,环境变异在33.21%~36.28%。因此,主基因和多基因共同决定了烟叶茄尼醇的含量,以主基因遗传为主,同时受到环境的影响。
To explore the genetic model of solanesol content of tobacco leaf, a cross was made with the low solanesol Maryland609 as maternal parent and the high solanesol K326 as paternal parent and populations of four generations （P1, P2, F1, F2） were obtained. Determination of solanesol content of the four populations was done with UPLC. Genetic analysis of solanesol content in tobacco was carried out using the joint segregation analysis method with a mixed genetic model of major gene plus polygene. The results showed that solanesol content of tobacco leaf appeared to be a quantitative trait and the inheritance fit to a mixed genetic model of two major genes with equal additive effects plus polygenes with additive-dominant effects （E6 model）. The results from two years showed that the additive effects and dominant effects of two major genes were equal and the quantitative value was negative. Dominant effects of polygenes were greater than additive effects. Heritabilities of the major genes were estimated to be 33.61% and 53.15%, respectively. Heritabilities of the polygenes were estimated to be 30.01% and 13.64%, respectively. The value of environmental variation was between 33.21%-36.28%. The results indicated that solanesol content of tobacco leaf was mainly controlled by major genes and poly genes, in which the major genes were dominant, and was effected by environmental factors at the same time.
出处
《中国烟草科学》
CSCD
北大核心
2015年第6期1-7,共7页
Chinese Tobacco Science
基金
公益性行业(农业)科研专项"烟草增香减害关键技术研究与示范"(201203091)
关键词
烟草
茄尼醇
主基因+多基因
遗传效应
tobacco
solanesol
major-gene plus polygenes
genetic effects
