摘要
目的:通过观察非甾体消炎药(Non-steroid anti-inflammatory drugs,NSAIDs)阿司匹林不同给药途径对大鼠小肠黏膜屏障及生长素抑素受体(Somatostatin receptors SSTR)水平的影响,探讨阿司匹林的最佳给药途径。方法:32只SD大鼠随机分为灌胃组、灌肠组、腹腔注射组及空白对照组四组,每组8只。对回肠黏膜损伤进行评分;ELISA检测各组回肠组织SSTR水平;免疫组化检测各组回肠组织SSTR的分布,图像分析软件测定IOD值。结果:(1)阿司匹林治疗组回肠黏膜损伤指数比正常对照组高(P<0.05),但各实验组之间两两比较黏膜损伤评分无差别;(2)各亚型SSTR在大鼠小肠黏膜均有表达,以SSRT1及SSTR2为主(P<0.05);(3)与空白对照组比较,实验组大鼠小肠黏膜中SSTR1-5表达均降低(P<0.05);(4)不同的给药方式对SSTR1-5的影响不完全一致,部分P<0.05。(5)四组间SST浓度、IOD值差异无统计学意义。结论:阿司匹林可以导致大鼠小肠黏膜的损伤。各亚型SSTR在大鼠小肠黏膜均有表达,阿司匹林可以导致小肠黏膜各型SSTR的表达下降,提示这种变化可能影响小肠黏膜的屏障功能。不同的给药途径对SSTR的影响不尽相同,提示我们使用阿司匹林时胃肠外给药可能会减少肠道损伤的发生。
Objective：To investigate the optimal administration approach of using aspirin by observing the effects of the rats intestinal mucosal barrier and somatostatin receptors level with different administration approaches of using non-steroid anti-inflammatory drugs-aspirin. Methods： 32 SD rats were randomly divided into 4 groups ： oral intake group, enema group, intraperitoneal injection group and control group. Every group had 8 rats. The ileum mucosal injury was scored. The level and distribution of SSTRs in every group was detected by ELISA and immunohistochemistry, respectively, The IOD score in each group was measured by image analysis. Results： （ 1 ） The scores of ileum mucosal injury in aspirin experimental groups were significantly higher than that in control group （ P〈0.05 ）, but there were no difference among aspirin experimental groups. （2） All subtypes of SSTR were expressed in rat intestinal mucosa. However,SSRT1 and SSTR2 were expressed mainly（P〈0. 05）. （3） The expressions of SSTR1-5 on intestinal mucosa in aspirin experimental groups were significantly less than that in control group （P〈0. 05 ）. （4） There was incompletely no same effects of SSTR1-5 in intestinal mucosa by different approaches （ P〈0.05 ）. （ 5 ） There were no significant difference of SST concentration and IOD score among all groups （ P〉0.05 ）. Conclusion： Aspirin can damage the rat intestinal mucosa. Aspirin could lead to the decrease of SSTRI-5 expression in the intestinal mucosa. All subtypes of SSTR in rat small intestine mucosa were expressed, suggesting that aspirin could affect the barrier function of the intestine. There were different influences on SSTR1-5 expression by different administration approaches, suggesting aspirin by parenteral approach may reduce the intestinal damage.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第2期178-183,共6页
Chinese Journal of Immunology
基金
贵州省科技厅基金(E2011-10)
