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方波脉冲电场杀灭微藻的实验研究 被引量:3

Experimental Study on Algae Inhibition Effect of Square Wave Pulsed Electric Field
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摘要 循环冷却水中的微藻繁殖会造成微生物污垢滋生,可采用电磁脉冲进行灭藻。为此,首先提出了一种基于Marx发生器和H桥固态调制器的高压脉冲源拓扑,并研制了一台多参数可调高压脉冲发生器,输出的方波脉冲的脉冲频率为1~1 000 s-1,脉冲电压0~6 k V,脉宽1~5μs,最大瞬时输出电流为12 A。同时,针对电场强度、脉宽、频率和处理时间这4个参量设计了正交实验方案,开展脉冲电场对蛋白核小球藻和铜绿微囊藻的灭藻实验。结果表明,脉冲电场处理对微藻生长具有显著抑制作用,且电场强度为抑制实验微藻生长的最重要因素,提高电场强度可以显著增强对微藻生长的抑制效果。此外,正交设计方案有助于以少量实验频次推测获得最佳的电磁脉冲处理参数,实验证实此脉冲源的最佳电参数组合分别为5 k V、5μs、800 Hz、5 min和5 k V、5μs、800 Hz、10 min,对应的藻细胞密度下降率分别为86.2%和94.1%。 Micro-algae reproducing abundantly in the circulating cooling water will lead to the breed of microbial fouling, and the electromagnetic pulse can be used to sterilize alga. Thus, we presented a topology circuit of high-voltage pulsed source based on Marx generator and H bridge solid state modulator. Then, we developed a multi-parameter tunable high-voltage pulser, and its output periodic square pulses has the pulse frequency of 1-1 000 s-1, the pulse voltage of 0-6 kV, the pulse width of 1-5μs, and the maximum instantaneous output current of 12 A. In addition, we designed an orthogonal experiment according to four parameters namely electric field intensity, pulse width, frequency, and treatment period, and then we carried out the algae destruction experiment of pulsed electric field on chlorella pyrenoidosa and microcystis aeruginosa. The results show that the pulsed electric field has a significant inhibitory effect on the growth of algae, and electric field intensity is the most important impact factor. Increasing electric field intensity can significantly enhance the inhibition effect on the growth of algae. Moreover, the orthogonal design is conducive to obtain the best electric pulse parameters through a few experiments. It is indicated that the optimum electric parameters of this pulser for growth inhibiting effect are 5 kV, 5 μs, 800 Hz, 5 min and 5 kV, 5 μs, 800 Hz, 10 min, and the descent rates of algal cell density are 86.2% and 94.1%, respectively.
出处 《高电压技术》 EI CAS CSCD 北大核心 2016年第2期368-376,共9页 High Voltage Engineering
基金 国家自然科学基金(51077139) 重庆市电器检测工程技术研究中心项目(CSTC2011pt-gc70009)~~
关键词 MARX发生器 脉冲电场 藻细胞密度 灭藻 正交实验设计 蛋白核小球藻 铜绿微囊藻 Marx generator pulsed electric field algae cell density algae inhibition orthogonal experimental design chlorella pyrenoidosa microcystis aeruginosa
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